Anti-SGCA Antibody (CAB4109)

Antibody Name:	Anti-SGCA Antibody
Antibody SKU:	CAB4109
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 60-290 of human SGCA (NP_000014.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, LO2, 293T, Mouse skeletal muscle, Mouse heart, Rat skeletal muscle, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 60-290 of human SGCA (NP_000014.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ITYH AHLQ GHPD LPRW LRYT QRSP HHPG FLYG SATP EDRG LQVI EVTA YNRD SFDT TRQR LVLE IGDP EGPL LPYQ AEFL VRSH DAEE VLPS TPAS RFLS ALGG LWEP GELQ LLNV TSAL DRGG RVPL PIEG RKEG VYIK VGSA SPFS TCLK MVAS PDSH ARCA QGQP PLLS CYDT LAPH FRVD WCNV TLVD KSVP EPAD EVPT PGDG ILEH DPFF CPPT EAPD RDFL VDA
Gene ID:	6442
Uniprot:	Q16586
Cellular Location:	Cell membrane, Cytoplasm, Single-pass type I membrane protein, cytoskeleton, sarcolemma
Calculated MW:	29kDa/42kDa
Observed MW:	43kDa

Synonyms:

SGCA, 50DAG, ADL, DAG2, DMDA2, LGMD2D, SCARMD1, adhalin

Background:

This gene encodes a component of the dystrophin-glycoprotein complex (DGC), which is critical to the stability of muscle fiber membranes and to the linking of the actin cytoskeleton to the extracellular matrix. Its expression is thought to be restricted to striated muscle. Mutations in this gene result in type 2D autosomal recessive limb-girdle muscular dystrophy. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	SGCA: Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. Defects in SGCA are the cause of limb-girdle muscular dystrophy type 2D (LGMD2D); also known as Duchenne- like muscular dystrophy autosomal recessive type 2 or severe childhood autosomal recessive muscular dystrophy (SCARMD). LGMD2D is an autosomal recessive degenerative myopathy characterized by progressive muscle wasting from early childhood with loss of independent ambulation by teenage years. Muscle biopsy shows necrosis, decreased immunostaining for alpha sarcoglycan, and adhalin deficiency. The phenotype is less severe than LGMD2C. Belongs to the sarcoglycan alpha/epsilon family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Membrane protein, integral Chromosomal Location of Human Ortholog: 17q21 Cellular Component: dystrophin-associated glycoprotein complex; sarcoglycan complex Molecular Function:protein binding Biological Process: muscle contraction; muscle development Disease: Muscular Dystrophy, Limb-girdle, Type 2d
NCBI Summary:	This gene encodes a component of the dystrophin-glycoprotein complex (DGC), which is critical to the stability of muscle fiber membranes and to the linking of the actin cytoskeleton to the extracellular matrix. Its expression is thought to be restricted to striated muscle. Mutations in this gene result in type 2D autosomal recessive limb-girdle muscular dystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
UniProt Code:	Q16586
NCBI GenInfo Identifier:	13431858
NCBI Gene ID:	6442
NCBI Accession:	Q16586.1
UniProt Secondary Accession:	Q16586,Q13710, Q13712, A6NEB8, A8K3K7,
UniProt Related Accession:	Q16586
Molecular Weight:	29,354 Da
NCBI Full Name:	Alpha-sarcoglycan
NCBI Synonym Full Names:	sarcoglycan alpha
NCBI Official Symbol:	SGCA
NCBI Official Synonym Symbols:	ADL; DAG2; 50DAG; DMDA2; LGMD2D; SCARMD1; adhalin
NCBI Protein Information:	alpha-sarcoglycan
UniProt Protein Name:	Alpha-sarcoglycan
UniProt Synonym Protein Names:	50 kDa dystrophin-associated glycoprotein; 50DAG; Adhalin; Dystroglycan-2
Protein Family:	Alpha-sarcoglycan
UniProt Gene Name:	SGCA
UniProt Entry Name:	SGCA_HUMAN

	Western blot analysis of extracts of various cell lines, using SGCA antibody (CAB4109) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using SGCA Rabbit pAb (CAB4109) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using SGCA Rabbit pAb (CAB4109) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of C6 cells using SGCA Rabbit pAb (CAB4109) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

SGCA Antibody (PACO05035)

SGCA Antibody (PACO12161)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)