The Delta-sarcoglycan (SGCD) Antibody (CAB13351) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and is validated for use in Western blot applications. By binding to the SGCD protein, this antibody allows for the detection and analysis of SGCD expression in various cell types, making it ideal for investigations in muscle biology and disease pathology.The SGCD gene encodes a protein called delta-sarcoglycan, which is part of a complex of proteins that maintain the structural integrity of muscle fibers. Mutations in the SGCD gene can lead to muscular dystrophy, a group of genetic disorders characterized by muscle weakness and degeneration.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Delta-sarcoglycan (SGCD) Antibody
SKU:
CAB13351
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Cell Membrane, Cytoplasm, Single-Pass Type Ii Membrane Protein, Cytoskeleton, Sarcolemma.
Calculated MW:
32kDa
Observed MW:
37-42kDa
The protein encoded by this gene is one of the four known components of the sarcoglycan complex, which is a subcomplex of the dystrophin-glycoprotein complex (DGC). DGC forms a link between the F-actin cytoskeleton and the extracellular matrix. This protein is expressed most abundantly in skeletal and cardiac muscle. Mutations in this gene have been associated with autosomal recessive limb-girdle muscular dystrophy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene.
Purification Method
Affinity purification
Gene ID
6444
RRID
AB_2760209
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Delta-sarcoglycan (SGCD) Rabbit pAb (CAB13351) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using Delta-sarcoglycan (SGCD) Rabbit pAb (CAB13351). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
