The SIGLEC5 Antibody (CAB4250) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications.Siglec5 is known to be involved in immune regulation and plays a role in modulating immune responses, particularly in the context of inflammation and autoimmune diseases. The antibody specifically binds to the Siglec5 protein, enabling its detection and analysis in various cell types.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
SIGLEC5 Antibody
SKU:
CAB4250
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CD170, OBBP2, CD33L2, OB-BP2, SIGLEC-5, SIGLEC5
Positive Sample:
Mouse spleen
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
61kDa
Observed MW:
70kDa
This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion.
Purification Method
Affinity purification
Gene ID
8778
RRID
AB_2765587
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse spleen, using SIGLEC5 Rabbit pAb (CAB4250) at 1:800 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
