The SIM2 Antibody (CAB15098) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit immunization, is highly specific to human samples and has been validated for use in applications such as Western blotting.SIM2, a transcriptional regulator involved in brain development and function, has been implicated in various neurological disorders, making it a promising target for research in neuroscience and neurodegenerative diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SIM2 Antibody
SKU:
CAB15098
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SIM, bHLHe15, HMC13F06, HMC29C01, SIM2
Positive Sample:
HepG2, PC-3, Mouse kidney
Cellular Localization:
Nucleus.
Calculated MW:
73kDa
Observed MW:
73kDa
This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
6493
RRID
AB_2761982
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using SIM2 Rabbit pAb (CAB15098) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
