Anti-SIM2 Antibody (CAB15098)

Antibody Name:	Anti-SIM2 Antibody
Antibody SKU:	CAB15098
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 342-525 of human SIM2 (NP_033664.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human
Positive Samples:	U-251MG, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 342-525 of human SIM2 (NP_033664.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LSLE QVST AKSQ DSWR TALS TSQE TRKL VKPK NTKM KTKL RTNP YPPQ QYSS FQMD KLEC GQLG NWRA SPPA SAAA PPEL QPHS ESSD LLYT PSYS LPFS YHYG HFPL DSHV FSSK KPML PAKF GQPQ GSPC EVAR FFLS TLPA SGEC QWHY ANPL VPSS SSPA KNPP EPPA NTAR HSLV PSYE
Gene ID:	6493
Uniprot:	Q14190
Cellular Location:	Nucleus
Calculated MW:	63kDa/73kDa
Observed MW:	56kDa

Synonyms:

SIM2, HMC13F06, HMC29C01, SIM, bHLHe15

Background:

This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants.

UniProt Protein Function:	SIM2: Transcription factor that may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; DNA-binding Chromosomal Location of Human Ortholog: 21q22.13 Cellular Component: nucleoplasm Molecular Function:signal transducer activity; DNA binding; protein heterodimerization activity; transcription factor activity Biological Process: nervous system development; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; embryonic pattern specification; cell differentiation; signal transduction; lung development
NCBI Summary:	This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014]
UniProt Code:	Q14190
NCBI GenInfo Identifier:	2851630
NCBI Gene ID:	6493
NCBI Accession:	Q14190.2
UniProt Related Accession:	Q14190
Molecular Weight:	73kDa
NCBI Full Name:	Single-minded homolog 2
NCBI Synonym Full Names:	SIM bHLH transcription factor 2
NCBI Official Symbol:	SIM2
NCBI Official Synonym Symbols:	SIM; bHLHe15; HMC13F06; HMC29C01
NCBI Protein Information:	single-minded homolog 2
UniProt Protein Name:	Single-minded homolog 2
UniProt Synonym Protein Names:	Class E basic helix-loop-helix protein 15; bHLHe15
Protein Family:	Single-minded
UniProt Gene Name:	SIM2
UniProt Entry Name:	SIM2_HUMAN

Western blot analysis of extracts of various cell lines, using SIM2 antibody (CAB15098) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Antibodies

SIM2 Antibody (PACO12212)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)