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CD172a/SIRPalpha Rabbit Monoclonal Antibody (CAB9545)

Datasheet MSDS
SKU CAB9545
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human, Mouse, Rat
Host Species Rabbit
Synonyms BIT, MFR, P84, SIRP, MYD-1, SHPS1, CD172A, PTPNS1, CD172a/SIRPα
Research Use Cell Biology
€131 - €457

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Description

system_update_altDatasheetsystem_update_altMSDS

CD172a/SIRPalpha Monoclonal Antibody (CAB9545)

The CD172a/SIRPalpha Monoclonal Antibody (CAB9545) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. It binds specifically to the SIRP Alpha protein, allowing for the detection and analysis of this important molecule in various cell types.SIRP Alpha is known to play a crucial role in immune cell communication and the regulation of immune responses. Its involvement in modulating immune reactions makes it a promising target for research in immunology, cancer biology, and inflammatory diseases.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:CD172a/SIRPalpha Monoclonal Antibody
SKU:CAB9545
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC1626
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:RIRQ KKAQ GSTS STRL HEPE KNAR EITQ DTND ITYA DLNL PKGK KPAP QAAE PNNH TEYA SIQT SPQP ASED TLTY ADLD MVHL NRTP KQPA PKPE PSFS E
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:1000 - 1:6000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:BIT, MFR, P84, SIRP, MYD-1, SHPS1, CD172A, PTPNS1, CD172a/SIRPα
Positive Sample:Mouse brain, Rat brain, THP-1, U-937
Cellular Localization:Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:55kDa
Observed MW:85kDa

The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene.

Purification MethodAffinity purification
Gene ID140885
RRIDAB_2863719
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using CD172a/SIRPα Rabbit mAb (CAB9545) at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Product ImageWestern blot analysis of various lysates using CD172a/SIRPα Rabbit mAb (CAB9545) at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
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