Anti-SIRT6 Antibody (CAB7416)

Antibody Name:	Anti-SIRT6 Antibody
Antibody SKU:	CAB7416
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of mouse SIRT6 (NP_001156902.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	HeLa, 293T, Mouse spleen, Mouse stomach, A-549, LO2, BT-474, HL-60, Mouse heart

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of mouse SIRT6 (NP_001156902.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NLQP TKHD RQAD LRIH GYVD EVMC RLMK HLGL EIPA WDGP CVLD KALP PLPR PVAL KAEP PVHL NGAV HVSY KSKP NSPI LHRP PKRV KTEA APS
Gene ID:	50721
Uniprot:	Q8N6T7
Cellular Location:	Nucleus, nucleoplasm
Calculated MW:	36kDa/39kDa
Observed MW:	39-45kDa

Synonyms:

SIRT6, SIR2L6, sirtuin 6

Background:

This gene encodes a member of the sirtuin family of NAD-dependent enzymes that are implicated in cellular stress resistance, genomic stability, aging and energy homeostasis. The encoded protein is localized to the nucleus, exhibits ADP-ribosyl transferase and histone deacetylase activities, and plays a role in DNA repair, maintenance of telomeric chromatin, inflammation, lipid and glucose metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	SIRT6: NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF- kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span. Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate. Interacts with RELA. Interacts with RBBP8; the interaction deacetylates RBBP8. Belongs to the sirtuin family. Class IV subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Deacetylase; EC 2.4.2.31; EC 3.5.1.-; Transferase Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nuclear telomeric heterochromatin; nucleolus; nucleus Molecular Function:NAD(P)+-protein-arginine ADP-ribosyltransferase activity; protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); zinc ion binding; NAD-dependent histone deacetylase activity; chromatin binding; NAD+ ADP-ribosyltransferase activity Biological Process: protein amino acid ADP-ribosylation; histone deacetylation
NCBI Summary:	This gene encodes a member of the sirtuin family of NAD-dependent enzymes that are implicated in cellular stress resistance, genomic stability, aging and energy homeostasis. The encoded protein is localized to the nucleus, exhibits ADP-ribosyl transferase and histone deacetylase activities, and plays a role in DNA repair, maintenance of telomeric chromatin, inflammation, lipid and glucose metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]
UniProt Code:	Q8N6T7
NCBI GenInfo Identifier:	38258612
NCBI Gene ID:	51548
NCBI Accession:	Q8N6T7.2
UniProt Related Accession:	Q8N6T7
Molecular Weight:	Observed MW: 39kDa
NCBI Full Name:	NAD-dependent protein deacetylase sirtuin-6
NCBI Synonym Full Names:	sirtuin 6
NCBI Official Symbol:	SIRT6
NCBI Official Synonym Symbols:	SIR2L6
NCBI Protein Information:	NAD-dependent protein deacetylase sirtuin-6
UniProt Protein Name:	NAD-dependent protein deacetylase sirtuin-6
UniProt Synonym Protein Names:	Regulatory protein SIR2 homolog 6; SIR2-like protein 6
UniProt Gene Name:	SIRT6
UniProt Entry Name:	SIR6_HUMAN

	Western blot analysis of extracts of various cell lines, using SIRT6 antibody (CAB7416) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Western blot analysis of extracts of various cell lines, using SIRT6 antibody (CAB7416) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies	ELISA Kits
Anti-SIRT6 Antibody (CAB18468)	Human SIRT6 ELISA Kit
Anti-SIRT6 Antibody (CAB3591)	Human NAD-dependent deacetylase sirtuin-6 (SIRT6) ELISA Kit
SIRT6 Antibody (PACO12222)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)