Anti-SLC18A3 Antibody (CAB16068)

Antibody Name:	Anti-SLC18A3 Antibody
Antibody SKU:	CAB16068
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human SLC18A3 (NP_003046.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human SLC18A3 (NP_003046.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MESA EPAG QARA AATK LSEA VGAA LQEP RRQR RLVL VIVC VALL LDNM LYMV IVPI VPDY IAHM RGGG EGPT RTPE VWEP TLPL PTPA NASA YTAN TSAS PTAA WPAG SALR PRYP TESE DVKI GVLF ASKA ILQL LVNP LSGP FIDR MS
Gene ID:	6572
Uniprot:	Q16572
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	56kDa
Observed MW:	65KDa

Synonyms:	SLC18A3, CMS21, VACHT
Background:	This gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.

UniProt Protein Function:	SLC18A3: Involved in acetylcholine transport into synaptic vesicles. Belongs to the major facilitator superfamily. Vesicular transporter family.
UniProt Protein Details:	Protein type:Transporter; Transporter, SLC family; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 10q11.2 Cellular Component: membrane; integral to plasma membrane; plasma membrane Molecular Function:acetylcholine transmembrane transporter activity Biological Process: synaptic transmission; acetylcholine transport; neurotransmitter secretion
NCBI Summary:	This gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q16572
NCBI GenInfo Identifier:	118582257
NCBI Gene ID:	6572
NCBI Accession:	NP_003046.2
UniProt Secondary Accession:	Q16572,B2R7S1,
UniProt Related Accession:	Q16572
Molecular Weight:	56,903 Da
NCBI Full Name:	vesicular acetylcholine transporter
NCBI Synonym Full Names:	solute carrier family 18 (vesicular acetylcholine transporter), member 3
NCBI Official Symbol:	SLC18A3
NCBI Official Synonym Symbols:	VACHT
NCBI Protein Information:	vesicular acetylcholine transporter
UniProt Protein Name:	Vesicular acetylcholine transporter
UniProt Synonym Protein Names:	Solute carrier family 18 member 3
Protein Family:	Vesicular acetylcholine transporter
UniProt Gene Name:	SLC18A3
UniProt Entry Name:	VACHT_HUMAN

	Western blot analysis of extracts of various cell lines, using SLC18A3 Polyclonal Antibody (CAB16068) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat brain using SLC18A3 antibody (CAB16068) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of mouse brain using SLC18A3 Rabbit pAb (CAB16068) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-SLC18A3 Antibody (CAB16067)	Human Vesicular acetylcholine transporter (SLC18A3) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)