Anti-SLC1A2 Antibody (CAB0910)

Antibody Name:	Anti-SLC1A2 Antibody
Antibody SKU:	CAB0910
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 495-574 of human SLC1A2 (NP_004162.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:5000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, HepG2, SW480, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 495-574 of human SLC1A2 (NP_004162.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HLSK SELD TIDS QHRV HEDI EMTK TQSI YDDM KNHR ESNS NQCV YAAH NSVI VDEC KVTL AANG KSAD CSVE EEPW KREK
Gene ID:	6506
Uniprot:	P43004
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	61kDa/62kDa
Observed MW:	65kDa

Synonyms:

SLC1A2, EAAT2, EIEE41, GLT-1, HBGT

Background:

This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Improper regulation of this gene is thought to be associated with several neurological disorders. Alternatively spliced transcript variants of this gene have been identified.

UniProt Protein Function:	Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
NCBI Summary:	This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Sep 2010]
UniProt Code:	P43004
NCBI GenInfo Identifier:	3041668
NCBI Gene ID:	6506
NCBI Accession:	P43004.2
UniProt Secondary Accession:	P43004,Q14417, Q541G6, B4DQE9, U3KQQ4,
UniProt Related Accession:	P43004
Molecular Weight:	61,008 Da
NCBI Full Name:	Excitatory amino acid transporter 2
NCBI Synonym Full Names:	solute carrier family 1 member 2
NCBI Official Symbol:	SLC1A2
NCBI Official Synonym Symbols:	HBGT; EAAT2; GLT-1
NCBI Protein Information:	excitatory amino acid transporter 2
UniProt Protein Name:	Excitatory amino acid transporter 2
UniProt Synonym Protein Names:	Glutamate/aspartate transporter II; Sodium-dependent glutamate/aspartate transporter 2; Solute carrier family 1 member 2
Protein Family:	Excitatory amino acid transporter
UniProt Gene Name:	SLC1A2
UniProt Entry Name:	EAA2_HUMAN

	Western blot analysis of extracts of various cell lines, using SLC1A2 antibody (CAB0910) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded rat pancreas using SLC1A2 antibody (CAB0910) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver using SLC1A2 antibody (CAB0910) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse pancreas using SLC1A2 antibody (CAB0910) at dilution of 1:100 (40x lens).

Antibodies

Anti-EAAT2 Antibody (CAB3679)

SLC1A2 Antibody (PACO07174)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)