Anti-SLC39A4 Antibody (CAB3454)

Antibody Name:	Anti-SLC39A4 Antibody
Antibody SKU:	CAB3454
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 23-327 of human SLC39A4 (NP_570901.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, 293T, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 23-327 of human SLC39A4 (NP_570901.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SPPA GLLS LLTS GQGA LDQE ALGG LLNT LADR VHCA NGPC GKCL SVED ALGL GEPE GSGL PPGP VLEA RYVA RLSA AAVL YLSN PEGT CEDA RAGL WASH ADHL LALL ESPK ALTP GLSW LLQR MQAR AAGQ TPKT ACVD IPQL LEEA VGAG APGS AGGV LAAL LDHV RSGS CFHA LPSP QYFV DFVF QQHS SEVP MTLA ELSA LMQR LGVG REAH SDHS HRHR GASS RDPV PLIS SSNS SSVW DTVC LSAR DVMA AYGL SEQA GVTP EAWA QLSP ALLQ QQLS GACT SQSR PPVQ DQLS QSER Y
Gene ID:	55630
Uniprot:	Q6P5W5
Cellular Location:	Cell membrane, Multi-pass membrane protein, Recycling endosome membrane
Calculated MW:	66kDa/68kDa
Observed MW:	75kDa

Synonyms:	SLC39A4, AEZ, AWMS2, ZIP4
Background:	This gene encodes a member of the zinc/iron-regulated transporter-like protein (ZIP) family. The encoded protein localizes to cell membranes and is required for zinc uptake in the intestine. Mutations in this gene result in acrodermatitis enteropathica. Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	SLC39A4: Plays an important role in cellular zinc homeostasis as a zinc transporter. Regulated in response to zinc availability. Defects in SLC39A4 are the cause of acrodermatitis enteropathica zinc-deficiency type (AEZ). AEZ is a rare autosomal recessive disease caused by the inability to absorb sufficient zinc. The clinical features are growth retardation, immune system dysfunction, alopecia, severe dermatitis, diarrhea and occasionally mental disorders. All these manifestations are reversible with zinc supplementation. Without zinc therapy this disease is fatal. Belongs to the ZIP transporter (TC 2.A.5) family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transporter, SLC family; Transporter; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: recycling endosome membrane; cytoplasmic membrane-bound vesicle; apical plasma membrane; integral to membrane; plasma membrane Molecular Function:zinc ion transmembrane transporter activity Biological Process: cellular zinc ion homeostasis; transmembrane transport Disease: Acrodermatitis Enteropathica, Zinc-deficiency Type
NCBI Summary:	This gene encodes a member of the zinc/iron-regulated transporter-like protein (ZIP) family. The encoded protein localizes to cell membranes and is required for zinc uptake in the intestine. Mutations in this gene result in acrodermatitis enteropathica. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]
UniProt Code:	Q6P5W5
NCBI GenInfo Identifier:	296452970
NCBI Gene ID:	55630
NCBI Accession:	Q6P5W5.3
UniProt Secondary Accession:	Q6P5W5,Q7L5S5, Q9H6T8, Q9NXC4,
UniProt Related Accession:	Q6P5W5
Molecular Weight:	647
NCBI Full Name:	Zinc transporter ZIP4
NCBI Synonym Full Names:	solute carrier family 39 (zinc transporter), member 4
NCBI Official Symbol:	SLC39A4
NCBI Official Synonym Symbols:	AEZ; ZIP4; AWMS2
NCBI Protein Information:	zinc transporter ZIP4; zrt- and Irt-like protein 4
UniProt Protein Name:	Zinc transporter ZIP4
UniProt Synonym Protein Names:	Solute carrier family 39 member 4; Zrt- and Irt-like protein 4; ZIP-4
Protein Family:	Zinc transporter
UniProt Gene Name:	SLC39A4
UniProt Entry Name:	S39A4_HUMAN

	Western blot analysis of extracts of various cell lines, using SLC39A4 antibody (CAB3454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using SLC39A4 antibody (CAB3454) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat liver using SLC39A4 antibody (CAB3454) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using SLC39A4 antibody (CAB3454) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
SLC39A4 Antibody (PACO02995)	Human ZIP4 (Solute carrier family 39 member 4) ELISA Kit (HUFI05573)
SLC39A4 Antibody (PACO12290)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)