The SMC3 Antibody (CAB18402) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to SMC3, researchers can accurately detect and analyze the protein in various cell types, making it suitable for studies in genetics, cell biology, and cancer research.SMC3 plays a crucial role in maintaining genomic stability by ensuring proper chromosome alignment and segregation during cell division.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
SMC3 Antibody
SKU:
CAB18402
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein.
Purification Method
Affinity purification
Gene ID
9126
RRID
AB_2862169
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using SMC3 Rabbit pAb (CAB18402) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunoprecipitation analysis of 600 μg extracts of Mouse spleen using 3 μg SMC3 antibody (CAB18402). Western blot was performed from the immunoprecipitate using SMC3 antibody (CAB18402) at a dilution of 1:1000.
