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Anti-SMYD4 Antibody (CAB2337)

SKU:
CAB2337
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • Cell Biology Antibodies 8 Anti-SMYD4 Antibody CAB2337
  • Cell Biology Antibodies 8 Anti-SMYD4 Antibody CAB2337
  • Cell Biology Antibodies 8 Anti-SMYD4 Antibody CAB2337
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-SMYD4 Antibody
Antibody SKU:CAB2337
Antibody Size:20uL, 50uL, 100uL
Application:WB IF IP
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human SMYD4 (NP_443160.2).
Application:WB IF IP
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:20 - 1:50
Reactivity:Human, Mouse
Positive Samples:MCF7, HeLa, Jurkat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human SMYD4 (NP_443160.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDLP VDEW KSYL LQKW ASLP TSVQ VTIS TAET LRDI FLHS SSLL QPED ELFL KRLS KGYL VGKD SDAP LFYR EEGN KKFQ EKDY TGAA VLYS KGVS HSRP NTED MSLC HANR SAAL FHLG QYET CLKD INRA QTHG YPER LQPK IMLR KAEC LVAL GRLQ EASQ TISD LERN FTAT PALA DVLP QTLQ RNLH RLKM KMQE KDSL TESF PAAL AKTL EDAA LREE NEQL SNAS SSIG LCVD PLKG RCLV ATKD ILPG ELLV
Gene ID:114826
Uniprot:Q8IYR2
Cellular Location:
Calculated MW:89kDa
Observed MW:90kDa
Synonyms:SMYD4, ZMYND21
Background:
UniProt Protein Function:SMYD4: Belongs to the class V-like SAM-binding methyltransferase superfamily
UniProt Protein Details:

Protein type:Methyltransferase, protein lysine, predicted

Chromosomal Location of Human Ortholog: 17p13.3

Molecular Function:methyltransferase activity; metal ion binding

Biological Process: methylation

UniProt Code:Q8IYR2
NCBI GenInfo Identifier:296452956
NCBI Gene ID:114826
NCBI Accession:Q8IYR2.3
UniProt Secondary Accession:Q8IYR2,Q8N1P2, Q8NAT0, Q96LV4, Q96PV2,
UniProt Related Accession:Q8IYR2
Molecular Weight:804
NCBI Full Name:SET and MYND domain-containing protein 4
NCBI Synonym Full Names:SET and MYND domain containing 4
NCBI Official Symbol:SMYD4  
NCBI Official Synonym Symbols:ZMYND21  
NCBI Protein Information:SET and MYND domain-containing protein 4
UniProt Protein Name:SET and MYND domain-containing protein 4
Protein Family:SET and MYND domain-containing protein
UniProt Gene Name:SMYD4  
UniProt Entry Name:SMYD4_HUMAN
Anti-SMYD4 Antibody (CAB2337)Western blot analysis of extracts of various cell lines, using SMYD4 antibody (CAB2337) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-SMYD4 Antibody (CAB2337)Immunofluorescence analysis of MCF-7 cells using SMYD4 antibody (CAB2337). Blue: DAPI for nuclear staining.
Anti-SMYD4 Antibody (CAB2337)Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug SMYD4 antibody (CAB2337). Western blot was performed from the immunoprecipitate using SMYD4 antibody (CAB2337) at a dilition of 1:500.____Detection: ECL Enhanced Kit (RM00021)._Exposure time: 20s.

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