The Slug Antibody (CAB1057) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, produced through immunization of rabbits, demonstrates high specificity for human samples and is suitable for use in Western blot analysis.Snai2, also known as Slug, plays a pivotal role in promoting EMT, a process involved in embryogenesis, wound healing, and cancer metastasis. Its dysregulation has been implicated in the progression and aggressiveness of various cancers, making it a promising target for therapeutic intervention.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Slug Antibody
SKU:
CAB1057
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SLUG, WS2D, SLUGH, SLUGH1, SNAIL2, Slug
Positive Sample:
HeLa, Mouse spleen
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
30kDa
Observed MW:
30kDa
This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects.
Purification Method
Affinity purification
Gene ID
6591
RRID
AB_2758111
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse spleen, using Slug Rabbit pAb (CAB1057) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Western blot analysis of lysates from HeLa cells, using Slug Rabbit pAb (CAB1057) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
