Anti-RAB13 Antibody (CAB10571)

Antibody Name:	Anti-RAB13 Antibody
Antibody SKU:	CAB10571
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-203 of human RAB13 (NP_002861.1).

Application:	WB IF
Recommended Dilution:	WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	HeLa, 293T, LO2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-203 of human RAB13 (NP_002861.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAKA YDHL FKLL LIGD SGVG KTCL IIRF AEDN FNNT YIST IGID FKIR TVDI EGKK IKLQ VWDT AGQE RFKT ITTA YYRG AMGI ILVY DITD EKSF ENIQ NWMK SIKE NASA GVER LLLG NKCD MEAK RKVQ KEQA DKLA REHG IRFF ETSA KSSM NVDE AFSS LARD ILLK SGGR RSGN GNKP PSTD LKTC DKKN TNKC SLG
Gene ID:	5872
Uniprot:	P51153
Cellular Location:	Cell junction, Cell membrane, Cell projection, Cytoplasmic side, Cytoplasmic vesicle, Cytoplasmic vesicle membrane, Golgi apparatus, Lipid-anchor, Recycling endosome membrane, lamellipodium, secretory vesicle membrane, tight junction, trans-Golgi network membrane
Calculated MW:	22kDa
Observed MW:	23kDa

Synonyms:

RAB13, GIG4

Background:

This gene is a member of the Rab family of small G proteins and plays a role in regulating membrane trafficking between trans-Golgi network (TGN) and recycling endosomes (RE). The encoded protein is involved in the assembly of tight junctions, which are components of the apical junctional complex (AJC) of epithelial cells. The AJC plays a role in forming a barrier between luminal contents and the underlying tissue. Additional functions associated with the protein include endocytic recycling of occludin, regulation of epithelial cell scattering, neuronal regeneration and regulation of neurite outgrowth. Alternately spliced transcript variants have been observed for this gene. A pseudogene associated with this gene is located on chromosome 12.

UniProt Protein Function:	RAB13: Could participate in polarized transport, in the assembly and/or the activity of tight junctions. Belongs to the small GTPase superfamily. Rab family.
UniProt Protein Details:	Protein type:G protein, monomeric, Rab; G protein, monomeric; G protein Chromosomal Location of Human Ortholog: 1q21.2 Cellular Component: cytoplasm; cytoplasmic vesicle; cytoplasmic vesicle membrane; endocytic vesicle; Golgi apparatus; lamellipodium; lateral plasma membrane; neuron projection; plasma membrane; recycling endosome; tight junction; trans-Golgi network Molecular Function:GTP binding; GTPase activity; protein binding Biological Process: cellular response to insulin stimulus; cortical actin cytoskeleton organization and biogenesis; endocytic recycling; endosome transport; neurite development; protein secretion; vesicle docking during exocytosis
NCBI Summary:	This gene is a member of the Rab family of small G proteins and plays a role in regulating membrane trafficking between trans-Golgi network (TGN) and recycling endosomes (RE). The encoded protein is involved in the assembly of tight junctions, which are components of the apical junctional complex (AJC) of epithelial cells. The AJC plays a role in forming a barrier between luminal contents and the underlying tissue. Additional functions associated with the protein include endocytic recycling of occludin, regulation of epithelial cell scattering, neuronal regeneration and regulation of neurite outgrowth. Alternately spliced transcript variants have been observed for this gene. A pseudogene associated with this gene is located on chromosome 12. [provided by RefSeq, Jan 2013]
UniProt Code:	P51153
NCBI GenInfo Identifier:	1710016
NCBI Gene ID:	5872
NCBI Accession:	P51153.1
UniProt Secondary Accession:	P51153,Q5U0A6, Q6GPG6, Q96GU4, A8K6B5, D3DV67,
UniProt Related Accession:	P51153
Molecular Weight:	22,774 Da
NCBI Full Name:	Ras-related protein Rab-13
NCBI Synonym Full Names:	RAB13, member RAS oncogene family
NCBI Official Symbol:	RAB13
NCBI Official Synonym Symbols:	GIG4
NCBI Protein Information:	ras-related protein Rab-13
UniProt Protein Name:	Ras-related protein Rab-13
UniProt Synonym Protein Names:	Cell growth-inhibiting gene 4 protein
Protein Family:	Ras-related protein
UniProt Gene Name:	RAB13
UniProt Entry Name:	RAB13_HUMAN

	Western blot analysis of extracts of various cell lines, using RAB13 antibody (CAB10571) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of U-2 OS cells using RAB13 Rabbit pAb (CAB10571) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)