Anti-SOCS6 Antibody (CAB9957)

Antibody Name:	Anti-SOCS6 Antibody
Antibody SKU:	CAB9957
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human SOCS6 (NP_004223.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	A-549, Mouse heart, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human SOCS6 (NP_004223.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MKKI SLKT LRKS FNLN KSKE ETDF MVVQ QPSL ASDF GKDD SLFG SCYG KDMA SCDI NGED EKGG KNRS KSES LMGT LKRR LSAK QKSK GKAG TPSG SSAD EDTF SSSS APIV FKDV RAQR PIRS TSLR SHHY SPAP WPLR PTNS EETC IKME VRVK ALVH SSSP SPAL NGVR KDFH DLQS ETTC QEQA NSLK SSAS HNGD LHLH LDEH VP
Gene ID:	9306
Uniprot:	O14544
Cellular Location:
Calculated MW:	59kDa
Observed MW:	70kDa

Synonyms:

SOCS6, CIS-4, CIS4, HSPC060, SOCS-4, SOCS-6, SOCS4, SSI4, STAI4, STATI4

Background:

The protein encoded by this gene contains a SH2 domain and a CIS homolog domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by GM-CSF and EPO in hematopoietic cells. A high expression level of this gene was found in factor-independent chronic myelogenous leukemia (CML) and erythroleukemia (HEL) cell lines.

UniProt Protein Function:	SOCS6: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. May be a substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Regulates KIT degradation by ubiquitination of the tyrosine-phosphorylated receptor.
UniProt Protein Details:	Protein type:Inhibitor Chromosomal Location of Human Ortholog: 18q22.2 Cellular Component: cytoplasm; immunological synapse Molecular Function:protein binding; protein kinase inhibitor activity Biological Process: cytokine and chemokine mediated signaling pathway; defense response; JAK-STAT cascade; negative regulation of JAK-STAT cascade; negative regulation of protein kinase activity; proteasomal protein catabolic process
NCBI Summary:	The protein encoded by this gene contains a SH2 domain and a CIS homolog domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by GM-CSF and EPO in hematopoietic cells. A high expression level of this gene was found in factor-independent chronic myelogenous leukemia (CML) and erythroleukemia (HEL) cell lines. [provided by RefSeq, Jul 2008]
UniProt Code:	O14544
NCBI GenInfo Identifier:	206729895
NCBI Gene ID:	9306
NCBI Accession:	O14544.2
UniProt Secondary Accession:	O14544,Q8WUM3,
UniProt Related Accession:	O14544
Molecular Weight:	59,528 Da
NCBI Full Name:	Suppressor of cytokine signaling 6
NCBI Synonym Full Names:	suppressor of cytokine signaling 6
NCBI Official Symbol:	SOCS6
NCBI Official Synonym Symbols:	CIS4; SSI4; CIS-4; SOCS4; STAI4; SOCS-4; SOCS-6; STATI4; HSPC060
NCBI Protein Information:	suppressor of cytokine signaling 6
UniProt Protein Name:	Suppressor of cytokine signaling 6
UniProt Synonym Protein Names:	Cytokine-inducible SH2 protein 4; CIS-4; Suppressor of cytokine signaling 4; SOCS-4
Protein Family:	Suppressor of cytokine signaling
UniProt Gene Name:	SOCS6
UniProt Entry Name:	SOCS6_HUMAN

	Western blot analysis of extracts of various cell lines, using SOCS6 antibody (CAB9957) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human mammary cancer using SOCS6 antibody (CAB9957) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver cancer using SOCS6 antibody (CAB9957) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using SOCS6 antibody (CAB9957) at dilution of 1:100 (40x lens).

Antibodies

SOCS6 Antibody (PACO12391)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)