Anti-SPAG8 Antibody (CAB8943)

Antibody Name:	Anti-SPAG8 Antibody
Antibody SKU:	CAB8943
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 220-420 of human SPAG8 (NP_758516.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	Mouse testis, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 220-420 of human SPAG8 (NP_758516.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LVAD RVPN YTSW SQHC PWEP QKQP PWEF LQVL EPGA RGLW KPPD IKGK LMVC YETL PRGQ CLLY NWEE ERAT NHLD QVPS MQDG SESF FFRH GHRG LLTM QLKS PMPS STTQ KDSY QPPG NVYW PLRG KREA MLEM LLQH QICK EVQA EQEP TRKL FEVE SVTH HDYR MELA QAGT PAPT KPHD YRQE QPET FWIQ RAPQ L
Gene ID:	26206
Uniprot:	Q99932
Cellular Location:	Cytoplasmic vesicle, acrosome, secretory vesicle
Calculated MW:	44kDa/51kDa/53kDa
Observed MW:	45kDa

Synonyms:

SPAG8, BS-84, CILD28, CT142, HSD-1, SMP1, SPAG3, hSMP-1

Background:

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. This protein interacts with ACT (activator of CREM in testis) and may play a role in CREM (cAMP response element modulator)-ACT-mediated gene transcription during spermatogenesis. This protein may also play a role in spermatogenesis by regulating microtubule formation and cell division. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.

UniProt Protein Function:	SPAG8: May play a role in fertility and microtubule formation through interaction with RANBP9. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cancer Testis Antigen (CTA) Chromosomal Location of Human Ortholog: 9p13.3 Cellular Component: cytoplasm; nucleus Molecular Function:protein binding Biological Process: positive regulation of transcription from RNA polymerase II promoter
NCBI Summary:	The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. This protein interacts with ACT (activator of CREM in testis) and may play a role in CREM (cAMP response element modulator)-ACT-mediated gene transcription during spermatogenesis. This protein may also play a role in spermatogenesis by regulating microtubule formation and cell division. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined. [provided by RefSeq, Jul 2012]
UniProt Code:	Q99932
NCBI GenInfo Identifier:	62511128
NCBI Gene ID:	26206
NCBI Accession:	Q99932.2
UniProt Secondary Accession:	Q99932,Q12937, Q5TCV8, Q8WWB4, B4DY89, E9PDV6,
UniProt Related Accession:	Q99932
Molecular Weight:	51,139 Da
NCBI Full Name:	Sperm-associated antigen 8
NCBI Synonym Full Names:	sperm associated antigen 8
NCBI Official Symbol:	SPAG8
NCBI Official Synonym Symbols:	SMP1; BS-84; CT142; HSD-1; SPAG3; CILD28; hSMP-1
NCBI Protein Information:	sperm-associated antigen 8
UniProt Protein Name:	Sperm-associated antigen 8
UniProt Synonym Protein Names:	HSD-1; Sperm membrane protein 1; SMP-1; Sperm membrane protein BS-84
Protein Family:	Sperm-associated antigen
UniProt Gene Name:	SPAG8
UniProt Entry Name:	SPAG8_HUMAN

Anti-SPAG8 Polyclonal Antibody (CAB8943)

Western blot analysis of extracts of various cell lines, using SPAG8 antibody (CAB8943) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

SPAG8 Antibody (PACO12416)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)