The SPC25 Antibody (CAB10653) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a protein that may be involved in kinetochore-microtubule interaction and spindle checkpoint activity. RRID AB_2772364 Gene ID 57405 Swiss Prot Synonym AD024; SPBC25; hSpc25; SPC25
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
SPC25 Antibody
SKU:
CAB10653
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AD024, SPBC25, hSpc25, SPC25
Positive Sample:
HeLa, PC-3
Cellular Localization:
Chromosome, Nucleus, Centromere, Kinetochore.
Calculated MW:
26kDa
Observed MW:
26kDa
This gene encodes a protein that may be involved in kinetochore-microtubule interaction and spindle checkpoint activity. RRID AB_2772364 Gene ID 57405 Swiss Prot Synonym AD024; SPBC25; hSpc25; SPC25
Purification Method:
Affinity purification
Gene ID:
57405
RRID:
AB_2772364
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using SPC25 Rabbit pAb (CAB10653) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
