Anti-SPINT1 Antibody (CAB6411)

Antibody Name:	Anti-SPINT1 Antibody
Antibody SKU:	CAB6411
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 234-513 of human SPINT1 (NP_003701.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 234-513 of human SPINT1 (NP_003701.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ANVT VTVL STKQ TEDY CLAS NKVG RCRG SFPR WYYD PTEQ ICKS FVYG GCLG NKNN YLRE EECI LACR GVQG PSME RRHP VCSG TCQP TQFR CSNG CCID SFLE CDDT PNCP DASD EAAC EKYT SGFD ELQR IHFP SDKG HCVD LPDT GLCK ESIP RWYY NPFS EHCA RFTY GGCY GNKN NFEE EQQC LESC RGIS KKDV FGLR REIP IPST GSVE MAVA VFLV ICIV VVVA ILGY CFFK NQRK DFHG HHHH PPPT PASS TVST TEDT EHLV YNHT TRPL
Gene ID:	6692
Uniprot:	O43278
Cellular Location:	Secreted
Calculated MW:	56kDa/58kDa
Observed MW:	70kDa

Synonyms:	SPINT1, HAI, HAI1, MANSC2
Background:	The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms.

UniProt Protein Function:	SPINT1: Inhibitor of HGF activator. Also acts as an inhibitor of matriptase (ST14). 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 15q15.1 Cellular Component: extracellular space; membrane; extracellular region Molecular Function:serine-type endopeptidase inhibitor activity Biological Process: extracellular matrix organization and biogenesis
NCBI Summary:	The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	O43278
NCBI GenInfo Identifier:	61252335
NCBI Gene ID:	6692
NCBI Accession:	O43278.2
UniProt Secondary Accession:	O43278,Q7Z7D2,
UniProt Related Accession:	O43278
Molecular Weight:	529
NCBI Full Name:	Kunitz-type protease inhibitor 1
NCBI Synonym Full Names:	serine peptidase inhibitor, Kunitz type 1
NCBI Official Symbol:	SPINT1
NCBI Official Synonym Symbols:	HAI; HAI1; MANSC2
NCBI Protein Information:	kunitz-type protease inhibitor 1; HAI-1; serine protease inhibitor, Kunitz type 1; hepatocyte growth factor activator inhibitor type 1
UniProt Protein Name:	Kunitz-type protease inhibitor 1
UniProt Synonym Protein Names:	Hepatocyte growth factor activator inhibitor type 1; HAI-1
Protein Family:	Kunitz-type protease inhibitor
UniProt Gene Name:	SPINT1
UniProt Entry Name:	SPIT1_HUMAN

	Western blot analysis of extracts of various cell lines, using SPINT1 antibody (CAB6411) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human prostate using SPINT1 antibody (CAB6411) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse esophageal cancer using SPINT1 antibody (CAB6411) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using SPINT1 antibody (CAB6411). Blue: DAPI for nuclear staining.

Antibodies

SPINT1 Antibody (PACO12436)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)