The SF1 Antibody (CAB6424) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.Splicing factor 1 is known to be involved in the splicing of precursor mRNA molecules, a crucial step in gene expression that can ultimately have a profound impact on protein diversity and cellular function. By targeting the splicing factor 1 protein, this antibody enables precise detection and analysis of splicing factor 1 in various cell types, making it an essential component in studies related to RNA processing and gene regulation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
SF1 Antibody
SKU:
CAB6424
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BBP, MBBP, ZFM1, ZNF162, D11S636, ZCCHC25, SF1
Positive Sample:
HepG2
Cellular Localization:
Nucleus.
Calculated MW:
68kDa
Observed MW:
68kDa
This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
7536
RRID
AB_2767026
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HepG2 cells, using SF1 Rabbit pAb (CAB6424) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
