Anti-SPTAN1 Antibody (CAB0160)

Antibody Name:	Anti-SPTAN1 Antibody
Antibody SKU:	CAB0160
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 950-1130 of human SPTAN1 (NP_001123910.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, Jurkat, A-549, NIH/3T3, Mouse kidney, Rat spinal cord

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 950-1130 of human SPTAN1 (NP_001123910.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	REQA QSCR QQVA PTDD ETGK ELVL ALYD YQEK SPRE VTMK KGDI LTLL NSTN KDWW KVEV NDRQ GFVP AAYV KKLD PAQS ASRE NLLE EQGS IALR QEQI DNQT RITK EAGS VSLR MKQV EELY HSLL ELGE KRKG MLEK SCKK FMLF REAN ELQQ WINE KEAA LTSE EVGA DLEQ VEVL Q
Gene ID:	6709
Uniprot:	Q13813
Cellular Location:	Cytoplasm, cell cortex, cytoskeleton
Calculated MW:	282kDa/284kDa/285kDa
Observed MW:	285kDa

Synonyms:

SPTAN1, EIEE5, NEAS, SPTA2

Background:

Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. This gene encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	SPTAN1: a cytoskeletal protein which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. Capable to forming dimers which can further associate to tetramers.
UniProt Protein Details:	Protein type:Cytoskeletal; Motility/polarity/chemotaxis; Adaptor/scaffold Chromosomal Location of Human Ortholog: 9q34.11 Cellular Component: cytosol; fascia adherens; intracellular membrane-bound organelle; lateral plasma membrane; membrane; microtubule cytoskeleton; protein complex; spectrin; Z disc Molecular Function:actin binding; calcium ion binding; calmodulin binding; protein binding; protein complex binding; protein heterodimerization activity; protein N-terminus binding; structural constituent of cytoskeleton; syntaxin binding Biological Process: actin cytoskeleton reorganization; actin filament capping; activation of MAPKK activity; apoptosis; axon guidance; cell structure disassembly during apoptosis; cellular protein metabolic process; epidermal growth factor receptor signaling pathway; ER to Golgi vesicle-mediated transport; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; nerve growth factor receptor signaling pathway; post-translational protein modification; programmed cell death; protein amino acid N-linked glycosylation via asparagine; Ras protein signal transduction; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway Disease: Epileptic Encephalopathy, Early Infantile, 5
NCBI Summary:	Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. This gene encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]
UniProt Code:	Q13813
NCBI GenInfo Identifier:	94730425
NCBI Gene ID:	6709
NCBI Accession:	Q13813.3
UniProt Secondary Accession:	Q13813,Q13186, Q15324, Q16606, Q59EF1, Q5VXV5, Q5VXV6 Q7Z6M5, Q9P0V0,
UniProt Related Accession:	Q13813
Molecular Weight:	282,282 Da
NCBI Full Name:	Spectrin alpha chain, non-erythrocytic 1
NCBI Synonym Full Names:	spectrin alpha, non-erythrocytic 1
NCBI Official Symbol:	SPTAN1
NCBI Official Synonym Symbols:	NEAS; EIEE5; SPTA2
NCBI Protein Information:	spectrin alpha chain, non-erythrocytic 1
UniProt Protein Name:	Spectrin alpha chain, non-erythrocytic 1
UniProt Synonym Protein Names:	Alpha-II spectrin; Fodrin alpha chain; Spectrin, non-erythroid alpha subunit
Protein Family:	Spectrin
UniProt Gene Name:	SPTAN1
UniProt Entry Name:	SPTN1_HUMAN

	Western blot analysis of extracts of various cell lines, using SPTAN1 antibody (CAB0160) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat liver using SPTAN1 antibody (CAB0160) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using SPTAN1 antibody (CAB0160) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using SPTAN1 antibody (CAB0160) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U-2 OS cells using SPTAN1 antibody (CAB0160) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Cleaved-SPTAN1 (D1185) Antibody (PACO06043)	Human SPTAN1 / Alpha-Fodrin ELISA Kit
	Human SPTAN1 (Alpha-Fodrin) CLIA Kit (HUES00873)
	Human Spectrin alpha chain, brain (SPTAN1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)