SQSTM1 / p62 Rabbit Polyclonal Antibody (CAB11250)

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CAB11250
  • Western blot analysis of extracts of various cell lines, using SQSTM1 antibody (CAB11250) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
  • Immunohistochemistry of paraffin-embedded human tonsil using SQSTM1 / p62 antibody (CAB11250) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using SQSTM1 / p62 antibody (CAB11250) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human tonsil using SQSTM1 / p62 antibody (CAB11250) at dilution of 1:150 (40x lens).
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using SQSTM1 / p62 antibody (CAB11250) at dilution of 1:150 (40x lens).
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Description

Technical Manual

SQSTM1 / p62 Rabbit Polyclonal Antibody

Product Name:SQSTM1 / p62 Rabbit Polyclonal Antibody
Product SKU:CAB11250
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human SQSTM1 / p62 (NP_001135770.1).
Sequence:

MAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL

Gene ID:8878
Swiss Prot:Q13501
Synonyms:SQSTM1; A170; DMRV; FTDALS3; NADGP; OSIL; PDB3; ZIP3; p60; p62; p62B
Calculated MW:38kDa/47kDa
Observed MW:62kDa
Reactivity:Human, Mouse, Rat
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation 
Positive Samples:BT-474, HeLa, Mouse heart
Cellular Location:Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Late endosome, Lysosome, Nucleus, P-body, autophagosome

Product Images

SQSTM1 / p62 Rabbit Polyclonal Antibody (CAB11250)

Western blot analysis of extracts of various cell lines, using SQSTM1 antibody (CAB11250) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

SQSTM1 / p62 Rabbit Polyclonal Antibody (CAB11250)

Immunohistochemistry of paraffin-embedded human tonsil using SQSTM1 / p62 antibody (CAB11250) at dilution of 1:100 (40x lens).

SQSTM1 / p62 Rabbit Polyclonal Antibody (CAB11250)

Immunohistochemistry of paraffin-embedded human colon carcinoma using SQSTM1 / p62 antibody (CAB11250) at dilution of 1:100 (40x lens).

SQSTM1 / p62 Rabbit Polyclonal Antibody (CAB11250)

Immunohistochemistry of paraffin-embedded human tonsil using SQSTM1 / p62 antibody (CAB11250) at dilution of 1:150 (40x lens).

SQSTM1 / p62 Rabbit Polyclonal Antibody (CAB11250)

Immunohistochemistry of paraffin-embedded human colon carcinoma using SQSTM1 / p62 antibody (CAB11250) at dilution of 1:150 (40x lens).

Uniprot Information

UniProt Protein Function:SQSTM1: an adapter protein which binds ubiquitin, shuttling proteins to the proteasome. Co-localizes along with the E3 ubiquitin ligase, TRAF6, to aggregates from Alzheimer's disease brains but not in control brain. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. Forms ternary complexes with PKCZ and Kv-beta2 or PKCZ and GABBR3. Also interacts with KCNAB1, GABRR1, GABRR2 and GABRR3. Interacts with EBI3, LCK, RASA1, PKCZ , PKCI, NR2F2, NTRK1, NTRK2, NTRK3, NBR1, MEK5, TRIM55 and MAPKAPK5. Interacts with the proteasome subunits PSMD4 and PSMC2. Interacts with K63- polyubiquitinated MAPT/TAU. Interacts with IKBKB through PRKCZ and PRKCI. Interacts with NGFR through TRAF6 and bridges that complex to NTRK1. Forms a complex with MEK5 and PRKCZ or PRKCI. Component of a ternary complex with PAWR and PRKCZ. Upon TNF-alpha stimulation, interacts with RIPK1 problably bridging IKBKB to the TNF-R1 complex composed of TNF-R1/TNFRSF1A, TRADD and RIPK1. Forms a complex with JUB/Ajuba, PRKCZ and TRAF6. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Nuclear receptor co-regulator; Autophagy; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: nucleoplasm; PML body; endoplasmic reticulum; lysosome; cytoplasm; late endosome; autophagic vacuole; cytoplasmic vesicle; inclusion body; cytosol

Molecular Function:identical protein binding; protein serine/threonine kinase activity; protein binding; ubiquitin binding; protein homodimerization activity; protein kinase C binding; zinc ion binding; SH2 domain binding; receptor tyrosine kinase binding; protein kinase binding

Biological Process: regulation of Ras protein signal transduction; ubiquitin-dependent protein catabolic process; nerve growth factor receptor signaling pathway; immune system process; positive regulation of apoptosis; protein heterooligomerization; macroautophagy; endosome transport; protein amino acid phosphorylation; protein localization; response to stress; autophagy; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; cell differentiation; regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of macroautophagy; negative regulation of apoptosis

Disease: Frontotemporal Dementia And/or Amyotrophic Lateral Sclerosis 3; Paget Disease Of Bone

NCBI Summary:This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]
UniProt Code:Q13501
NCBI GenInfo Identifier:74735628
NCBI Gene ID:8878
NCBI Accession:Q13501.1
UniProt Related Accession:Q13501
Molecular Weight:
NCBI Full Name:Sequestosome-1
NCBI Synonym Full Names:sequestosome 1
NCBI Official Symbol:SQSTM1 
NCBI Official Synonym Symbols:p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3 
NCBI Protein Information:sequestosome-1
UniProt Protein Name:Sequestosome-1
UniProt Synonym Protein Names:EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62
UniProt Gene Name:SQSTM1 
UniProt Entry Name:SQSTM_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
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