The SRSF2 Antibody (CAB3635) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced by Assay Genie through immunization of rabbits and is highly specific to human samples. Validated for use in Western blot applications, this antibody binds specifically to the SRSF2 protein, allowing for accurate detection and analysis in a variety of cell types.SRSF2 is a member of the serine/arginine-rich splicing factor family and plays a crucial role in pre-mRNA splicing, alternative splicing, and mRNA export. Dysregulation of SRSF2 has been linked to various diseases, including cancer, neurodevelopmental disorders, and viral infections.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SRSF2 Antibody
SKU:
CAB3635
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SC35, PR264, SC-35, SFRS2, SFRS2A, SRp30b, SRSF2
Positive Sample:
Mouse lung, Raji, THP-1
Cellular Localization:
Nucleus, Nucleus Speckle, Nucleoplasm.
Calculated MW:
25kDa
Observed MW:
28kDa/32kDa
The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding the same protein and one non-coding transcript variant have been found for this gene. In addition, a pseudogene of this gene has been found on chromosome 11.
Purification Method
Affinity purification
Gene ID
6427
RRID
AB_2765195
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse lung, using SRSF2 Rabbit pAb (CAB3635) at 1:1000 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 90s.
Western blot analysis of various lysates using SRSF2 Rabbit pAb (CAB3635) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human lung using SRSF2 Rabbit pAb (CAB3635) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
