Anti-SRSF3 Antibody (CAB6067)

Antibody Name:	Anti-SRSF3 Antibody
Antibody SKU:	CAB6067
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human SRSF3 (NP_003008.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HeLa, K-562, NIH/3T3, PC-12, Mouse brain, Mouse thymus, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human SRSF3 (NP_003008.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MHRD SCPL DCKV YVGN LGNN GNKT ELER AFGY YGPL RSVW VARN PPGF AFVE FEDP RDAA DAVR ELDG RTLC GCRV RVEL SNGE KRSR NR
Gene ID:	6428
Uniprot:	P84103
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	14kDa/19kDa
Observed MW:	22kDa

Synonyms:

SRSF3, SFRS3, SRp20

Background:

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants, one protein-coding and the other non-coding, have been found for this gene.

UniProt Code:	P84103
NCBI GenInfo Identifier:	51338672
NCBI Gene ID:	6428
NCBI Accession:	P84103.1
UniProt Secondary Accession:	P84103,O08831, P23152, Q5R3K0, B4E241,
UniProt Related Accession:	P84103
Molecular Weight:	164
NCBI Full Name:	Serine/arginine-rich splicing factor 3
NCBI Synonym Full Names:	serine/arginine-rich splicing factor 3
NCBI Official Symbol:	SRSF3
NCBI Official Synonym Symbols:	SFRS3; SRp20
NCBI Protein Information:	serine/arginine-rich splicing factor 3; pre-mRNA-splicing factor SRP20; splicing factor, arginine/serine-rich 3; splicing factor, arginine/serine-rich, 20-kD
UniProt Protein Name:	Serine/arginine-rich splicing factor 3
UniProt Synonym Protein Names:	Pre-mRNA-splicing factor SRP20; Splicing factor, arginine/serine-rich 3
Protein Family:	Serine/arginine-rich splicing factor
UniProt Gene Name:	SRSF3
UniProt Entry Name:	SRSF3_HUMAN

	Western blot analysis of extracts of various cell lines, using SRSF3 antibody (CAB6067) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded Rat brain using SRSF3 antibody (CAB6067) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human mammary cancer using SRSF3 antibody (CAB6067) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Mouse testis using SRSF3 antibody (CAB6067) at dilution of 1:100 (20x lens).
	Immunofluorescence analysis of C6 cells using SRSF3 antibody (CAB6067) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-SRSF3 Antibody (CAB9054)	Human SRSF3 / Serine / arginine-rich splicing factor 3 ELISA Kit
SRSF3 Antibody (PACO07150)	Human Serine/arginine-rich splicing factor 3 (SRSF3) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)