Anti-ST3GAL3 Antibody (CAB6753)

Antibody Name:	Anti-ST3GAL3 Antibody
Antibody SKU:	CAB6753
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-375 of human ST3GAL3 (NP_006270.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse skeletal muscle, Mouse heart, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-375 of human ST3GAL3 (NP_006270.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LHLL QWEE DSNS VVLS FDSA GQTL GSEY DRLG FLLN LDSK LPAE LATK YANF SEGA CKPG YASA LMTA IFPR FSKP APMF LDDS FRKW ARIR EFVP PFGI KGQD NLIK AILS VTKE YRLT PALD SLRC RRCI IVGN GGVL ANKS LGSR IDDY DIVV RLNS APVK GFEK DVGS KTTL RITY PEGA MQRP EQYE RDSL FVLA GFKW QDFK WLKY IVYK ERVS ASDG FWKS VATR VPKE PPEI RILN PYFI QEAA FTLI GLPF NNGL MGRG NIPT LGSV AVTM ALHG CDEV AVAG FGYD MSTP NAPL HYYE TVRM AAIK ESWT HNIQ REKE FLRK LVKA RVIT DLSS GI
Gene ID:	6487
Uniprot:	Q11203
Cellular Location:	Golgi apparatus, Golgi stack membrane, Secreted, Single-pass type II membrane protein
Calculated MW:	5kDa/8kDa/11-49kDa
Observed MW:	42kDa

Synonyms:

ST3GAL3, EIEE15, MRT12, SIAT6, ST3GALII, ST3GalIII, ST3N

Background:

The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi apparatus but can be proteolytically processed to a soluble form. This protein is a member of glycosyltransferase family 29. Mutations in this gene have been associated with autosomal recessive nonsymdromic mental retardation-12 (MRT12). Multiple transcript variants encoding several different isoforms have been found for this gene.

UniProt Protein Function:	ST3GAL3: Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta- 1,4-GlcNAc-, NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- or NeuAc-alpha- 2,3-Gal-beta-1,3-GalNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. The highest activity is toward Gal-beta-1,3-GlcNAc and the lowest toward Gal-beta-1,3- GalNAc. Defects in ST3GAL3 are the cause of mental retardation autosomal recessive type 12 (MRT12). Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Belongs to the glycosyltransferase 29 family. 26 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Transferase; EC 2.4.99.6; Membrane protein, integral; Glycan Metabolism - keratan sulfate biosynthesis Chromosomal Location of Human Ortholog: 1pter-p32.3 Disease: Mental Retardation, Autosomal Recessive 12
NCBI Summary:	The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi apparatus but can be proteolytically processed to a soluble form. This protein is a member of glycosyltransferase family 29. Mutations in this gene have been associated with autosomal recessive nonsymdromic mental retardation-12 (MRT12). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]
UniProt Code:	Q11203
NCBI GenInfo Identifier:	1705561
NCBI Gene ID:	6487
NCBI Accession:	Q11203.1
UniProt Secondary Accession:	Q11203,Q5T4W9, Q5T4X0, Q5T4X7, Q5T4X8, Q5T4X9, Q5T4Y0 Q5T4Y2, Q5T4Y3, Q5T4Y4, A9Z1W2, D3DPX8,
UniProt Related Accession:	Q11203
Molecular Weight:	8,178 Da
NCBI Full Name:	CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase
NCBI Synonym Full Names:	ST3 beta-galactoside alpha-2,3-sialyltransferase 3
NCBI Official Symbol:	ST3GAL3
NCBI Official Synonym Symbols:	ST3N; MRT12; SIAT6; EIEE15; ST3GALII; ST3GalIII
NCBI Protein Information:	CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase; ST3Gal III; alpha 2,3-ST 3; alpha-2,3-sialyltransferase II; alpha 2,3-sialyltransferase III; Gal beta-1,3(4)GlcNAc alpha-2,3 sialyltransferase; mental retardation, non-syndromic, autosomal recessive, 12; sialyltransferase 6 (N-acetyllacosaminide alpha 2,3-sialyltransferase)
UniProt Protein Name:	CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase
UniProt Synonym Protein Names:	Beta-galactoside alpha-2,3-sialyltransferase 3; Alpha 2,3-ST 3; Gal beta-1,3(4) GlcNAc alpha-2,3 sialyltransferase; N-acetyllactosaminide alpha-2,3-sialyltransferase; ST3Gal III; ST3GalIII; ST3N; Sialyltransferase 6
Protein Family:	CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase
UniProt Gene Name:	ST3GAL3
UniProt Entry Name:	SIAT6_HUMAN

	Western blot analysis of extracts of various cell lines, using ST3GAL3 antibody (CAB6753) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver damage using ST3GAL3 antibody (CAB6753) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat lung using ST3GAL3 antibody (CAB6753) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spinal cord using ST3GAL3 antibody (CAB6753) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using ST3GAL3 antibody (CAB6753). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)