Western blot analysis of extracts of various cell lines, using AMSH antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
The AMSH Monoclonal Antibody (CAB20889) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and has high specificity for human samples, making it ideal for use in Western blot applications to detect and analyze AMSH protein levels.AMSH, also known as associated molecule with the SH3 domain of STAM, is a crucial player in the process of endosomal trafficking and protein turnover. Dysregulation of AMSH activity has been linked to various diseases, including cancer and neurological disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Monkey samples.
Product Name:
AMSH Monoclonal Antibody
SKU:
CAB20889
Size:
20μL, 100μL
Reactivity:
Human, Monkey
Clone Number:
ARC2840
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
FPKA EELK AELL KRYT KEYT EYNE EKKK EAEE LARN MAIQ QELE KEKQ RVAQ QKQQ QLEQ EQFH AFEE MIRN QELE KERL KIVQ EFGK VDPG LGGP LVPD L
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AMSH, MICCAP
Positive Sample:
293T, HeLa, Jurkat, COS-7
Cellular Localization:
Cytoplasm, Early Endosome, Membrane, Nucleus, Peripheral Membrane Protein.
Calculated MW:
48kDa
Observed MW:
50kDa
Cytokine-mediated signal transduction in the JAK-STAT cascade requires the involvement of adaptor molecules. One such signal-transducing adaptor molecule contains an SH3 domain that is required for induction of MYC and cell growth. The protein encoded by this gene binds to the SH3 domain of the signal-transducing adaptor molecule, and plays a critical role in cytokine-mediated signaling for MYC induction and cell cycle progression. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.
Purification Method
Affinity purification
Gene ID
10617
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using AMSH Rabbit mAb (CAB20889) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
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