STAR Rabbit Polyclonal Antibody (CAB1035)

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CAB1035
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Description

Technical Manual

STAR Rabbit Polyclonal Antibody

Product Name:STAR Rabbit Polyclonal Antibody
Product SKU:CAB1035
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13.

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 64-285 of human STAR (NP_000340.2).
Sequence:

EETLYSDQELAYLQQGEEAMQKALGILSNQEGWKKESQQDNGDKVMSKVVPDVGKVFRLEVVVDQPMERLYEELVERMEAMGEWNPNVKEIKVLQKIGKDTFITHELAAEAAGNLVGPRDFVSVRCAKRRGSTCVLAGMATDFGNMPEQKGVIRAEHGPTCMVLHPLAGSPSKTKLTWLLSIDLKGWLPKSIINQVLSQTQVDFANHLRKRLESHPASEARC

Gene ID:6770
Swiss Prot:P49675
Synonyms:STAR; STARD1; STAR
Calculated MW:31kDa
Observed MW:28kDa
Reactivity:Human, Mouse
Application:WB IP
Recommended Dilution:WB 1:500 - 1:2000 IP 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunoprecipitation 
Positive Samples:Jurkat
Cellular Location:Mitochondrion

Product Images

STAR Rabbit Polyclonal Antibody (CAB1035)

Western blot analysis of extracts of various cell lines, using STAR antibody (CAB1035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).

Uniprot Information

UniProt Protein Function:STAR: Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone. May interact with TSPO. Expressed in gonads, adrenal cortex and kidney.
UniProt Protein Details:

Protein type:Mitochondrial; Lipid-binding; Carrier

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: mitochondrial crista; neuron projection; cell soma; mitochondrial intermembrane space; cytosol

Molecular Function:cholesterol transporter activity; cholesterol binding

Biological Process: response to nicotine; steroid metabolic process; diterpenoid metabolic process; dibenzo-p-dioxin metabolic process; regulation of steroid biosynthetic process; estrogen biosynthetic process; biphenyl metabolic process; circadian sleep/wake cycle, REM sleep; negative regulation of neuron apoptosis; response to nutrient; response to corticosterone stimulus; response to drug; cholesterol metabolic process; bile acid biosynthetic process; positive regulation of neurogenesis; insecticide metabolic process; progesterone biosynthetic process; fractalkine metabolic process; male gonad development; phthalate metabolic process; intracellular cholesterol transport; response to herbicide; glucocorticoid metabolic process; response to ethanol; response to hydrogen peroxide; cellular response to insulin stimulus; response to estrogen stimulus; response to lead ion; C21-steroid hormone biosynthetic process; brain development; response to activity; response to ionizing radiation; steroid biosynthetic process; regulation of neuronal synaptic plasticity; phenol metabolic process

Disease: Lipoid Congenital Adrenal Hyperplasia

NCBI Summary:The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13. [provided by RefSeq, Jul 2008]
UniProt Code:P49675
NCBI GenInfo Identifier:71152974
NCBI Gene ID:6770
NCBI Accession:P49675.2
UniProt Secondary Accession:P49675,Q16396,
UniProt Related Accession:P49675
Molecular Weight:285
NCBI Full Name:Steroidogenic acute regulatory protein, mitochondrial
NCBI Synonym Full Names:steroidogenic acute regulatory protein
NCBI Official Symbol:STAR 
NCBI Official Synonym Symbols:STARD1 
NCBI Protein Information:steroidogenic acute regulatory protein, mitochondrial; cholesterol trafficker; START domain containing 1; steroid acute regulatory protein; START domain-containing protein 1; mitochondrial steroid acute regulatory protein; StAR-related lipid transfer (START) domain containing 1
UniProt Protein Name:Steroidogenic acute regulatory protein, mitochondrial
UniProt Synonym Protein Names:START domain-containing protein 1; StARD1
Protein Family:Starch synthase
UniProt Gene Name:STAR 
UniProt Entry Name:STAR_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
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