Anti-STAT1 Antibody (CAB12075)

Antibody Name:	Anti-STAT1 Antibody
Antibody SKU:	CAB12075
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 650-750 of human STAT1 (NP_009330.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 650-750 of human STAT1 (NP_009330.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NYKV MAAE NIPE NPLK YLYP NIDK DHAF GKYY SRPK EAPE PMEL DGPK GTGY IKTE LISV SEVH PSRL QTTD NLLP MSPE EFDE VSRI VGSV EFDS MMNT V
Gene ID:	6772
Uniprot:	P42224
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	83kDa/87kDa
Observed MW:	91kDa

Synonyms:

CANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91, STAT1 alpha, STAT1

Background:

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.

UniProt Protein Function:	STAT1: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of certain receptor tyrosine kinases, GPCRs, and receptors for various interleukins and interferons. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 2q32.2 Cellular Component: axon; cytoplasm; cytosol; dendrite; nuclear chromatin; nucleolus; nucleoplasm; nucleus; perinuclear region of cytoplasm Molecular Function:double-stranded DNA binding; enzyme binding; identical protein binding; protein binding; protein homodimerization activity; signal transducer activity; transcription factor activity; tumor necrosis factor receptor binding Biological Process: apoptosis; blood circulation; defense response to virus; endothelial cell migration; JAK-STAT cascade; negative regulation of angiogenesis; negative regulation of endothelial cell proliferation; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transcription from RNA polymerase II promoter; negative regulation of viral protein levels in host cell; positive regulation of mesenchymal cell proliferation; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of apoptosis; regulation of transcription from RNA polymerase II promoter; response to cAMP; response to cytokine stimulus; response to peptide hormone stimulus; transcription, DNA-dependent; tumor necrosis factor-mediated signaling pathway; viral reproduction Disease: Immunodeficiency 31a; Immunodeficiency 31b; Immunodeficiency 31c
NCBI Summary:	The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:	P42224
NCBI GenInfo Identifier:	2507413
NCBI Gene ID:	6772
NCBI Accession:	P42224.2
UniProt Secondary Accession:	P42224,Q53S88, Q53XW4, Q68D00, Q9UDL5, A8K989, B2RCA0 D2KFR8, D3DPI7,
UniProt Related Accession:	P42224
Molecular Weight:
NCBI Full Name:	Signal transducer and activator of transcription 1-alpha/beta
NCBI Synonym Full Names:	signal transducer and activator of transcription 1
NCBI Official Symbol:	STAT1
NCBI Official Synonym Symbols:	CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
NCBI Protein Information:	signal transducer and activator of transcription 1-alpha/beta
UniProt Protein Name:	Signal transducer and activator of transcription 1-alpha/beta
UniProt Synonym Protein Names:	Transcription factor ISGF-3 components p91/p84
Protein Family:	Signal transducer and activator of transcription
UniProt Gene Name:	STAT1
UniProt Entry Name:	STAT1_HUMAN

	Western blot analysis of extracts of HeLa cells, using STAT1 antibody (CAB12075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat lymph node using STAT1 antibody (CAB12075) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using STAT1 antibody (CAB12075) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using STAT1 antibody (CAB12075) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using STAT1 Rabbit pAb (CAB12075) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-STAT1 Antibody (CAB0027)	Human STAT1 ELISA Kit
Anti-STAT1 Mouse Monoclonal Antibody (CAB10100)	Human Signal transducer and activator of transcription 1-alpha/beta (STAT1) ELISA Kit
Anti-STAT1 Antibody (CAB12330)	STAT1 (Phospho-Ser727) Transcription Factor Activity Assay
Anti-Phospho-STAT1-S727 Antibody (CABP0109)	STAT1 (Phospho-Tyr701) Transcription Factor Activity Assay
Anti-Phospho-STAT1-Y701 Antibody (CABP0135)	STAT1 Transcription Factor Activity Assay

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)