Anti-STAT4 Antibody (CAB6991)

Antibody Name:	Anti-STAT4 Antibody
Antibody SKU:	CAB6991
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 569-748 of human STAT4 (NP_003142.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	NCI-H460

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 569-748 of human STAT4 (NP_003142.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	WIDG YVMG FVSK EKER LLLK DKMP GTFL LRFS ESHL GGIT FTWV DHSE SGEV RFHS VEPY NKGR LSAL PFAD ILRD YKVI MAEN IPEN PLKY LYPD IPKD KAFG KHYS SQPC EVSR PTER GDKG YVPS VFIP ISTI RSDS TEPH SPSD LLPM SPSV YAVL RENL SPTT IETA MKSP YSAE
Gene ID:	6775
Uniprot:	Q14765
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	85kDa
Observed MW:	76kDa

Synonyms:

STAT4, SLEB11

Background:

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein.

UniProt Protein Function:	STAT4: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells.
UniProt Protein Details:	Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 2q32.2-q32.3 Cellular Component: nucleoplasm; cytoplasm Molecular Function:protein binding; signal transducer activity; sequence-specific DNA binding; transcription factor activity Biological Process: cell proliferation; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of transcription from RNA polymerase II promoter; JAK-STAT cascade; protein amino acid phosphorylation Disease: Systemic Lupus Erythematosus, Susceptibility To, 11
NCBI Summary:	The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]
UniProt Code:	Q14765
NCBI GenInfo Identifier:	6226158
NCBI Gene ID:	6775
NCBI Accession:	Q14765.1
UniProt Secondary Accession:	Q14765,Q96NZ6,
UniProt Related Accession:	Q14765
Molecular Weight:	85,941 Da
NCBI Full Name:	Signal transducer and activator of transcription 4
NCBI Synonym Full Names:	signal transducer and activator of transcription 4
NCBI Official Symbol:	STAT4
NCBI Official Synonym Symbols:	SLEB11
NCBI Protein Information:	signal transducer and activator of transcription 4
UniProt Protein Name:	Signal transducer and activator of transcription 4
Protein Family:	Protein slender lobes
UniProt Gene Name:	STAT4
UniProt Entry Name:	STAT4_HUMAN

	Western blot analysis of extracts of NCI-H460 cells, using STAT4 antibody (CAB6991) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat spleen using STAT4 antibody (CAB6991) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human rectum using STAT4 antibody (CAB6991) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using STAT4 antibody (CAB6991) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF7 cells using STAT4 antibody (CAB6991). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-STAT4 Antibody (CAB2638)	Human STAT4 ELISA Kit
Anti-Phospho-STAT4-Y693 Antibody (CABP0137)	Human STAT4 (Signal Transducer and Activator of Transcription 4) CLIA Kit (HUES01132)
Anti-Phospho-STAT4-Y693 pAb Antibody (CABP0781)	Human Signal transducer and activator of transcription 4 (STAT4) ELISA Kit
Anti-STAT4 Antibody (CAB4523)	STAT4 (Phospho-Tyr693) Colorimetric Cell-Based ELISA Kit
Phospho-STAT4 (Y693) Antibody (PACO00268)	STAT4 Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)