The STAT5A Monoclonal Antibody (CAB21228) is a high-quality antibody developed for reliable detection and analysis of target proteins. STAT5A is a key player in various cellular processes, including cell growth, differentiation, and survival. This monoclonal antibody, produced through hybridoma technology, is highly specific to human STAT5A and is suitable for use in Western blot and immunohistochemistry applications.By targeting the STAT5A protein, this antibody enables researchers to examine the activation and expression levels of STAT5A in different cell types and tissues.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
STAT5A Monoclonal Antibody
SKU:
CAB21228
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC52814
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MGF, STAT5, STAT5A
Positive Sample:
K-562, PC-3, HeLa, Mouse liver
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
91kDa
Observed MW:
91kDa
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene.
Purification Method
Affinity purification
Gene ID
6776
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using STAT5A Rabbit mAb (CAB21228) at 1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
