The STUB1 Antibody (CAB11751) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated using rabbit immunization, exhibits high specificity and sensitivity towards STUB1 in human samples, making it suitable for Western blot applications.STUB1, also known as CHIP, is a chaperone-dependent E3 ubiquitin ligase that plays a crucial role in regulating protein quality control and turnover. Dysregulation of STUB1 has been implicated in various diseases, including neurodegenerative disorders and cancer.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
STUB1 Antibody
SKU:
CAB11751
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
HeLa, HT-29, OVCAR3, HT-1080, Mouse brain, Mouse kidney, Rat brain, Rat heart
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
35kDa
Observed MW:
34kDa
This gene encodes a protein containing tetratricopeptide repeat and a U-box that functions as a ubiquitin ligase/cochaperone. The encoded protein binds to and ubiquitinates shock cognate 71 kDa protein (Hspa8) and DNA polymerase beta (Polb), among other targets. Mutations in this gene cause spinocerebellar ataxia, autosomal recessive 16. Alternative splicing results in multiple transcript variants. There is a pseudogene for this gene on chromosome 2.
Purification Method
Affinity purification
Gene ID
10273
RRID
AB_2758735
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using STUB1 Rabbit pAb (CAB11751) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of C6 cells using STUB1 Rabbit pAb (CAB11751) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using STUB1 Rabbit pAb (CAB11751) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western blot analysis of various lysates using STUB1 Rabbit pAb (CAB11751) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
