Anti-STXBP2 Antibody (CAB12511)

Antibody Name:	Anti-STXBP2 Antibody
Antibody SKU:	CAB12511
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human STXBP2 (NP_001120868.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	THP-1, Jurkat, Mouse testis, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human STXBP2 (NP_001120868.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAPS GLKA VVGE KILS GVIR SVKK DGEW KVLI MDHP SMRI LSSC CKMS DILA EGIT IVED INKR REPI PSLE AIYL LSPT EKAL IKDF QGTP TFTY KAAH IFFT DTCP EPLF SELG RSRL AKVV KTLK EIHL AFLP YEAQ VFSL DAPH STYN LYCP FRAE ERTR QLEV LAQQ IATL CATL QEYP AIRY RKGP EDTA QLAH AVLA KLNA FKAD TPSL GEGP EKTR SQLL IMDR AADP VSPL LHEL TFQA MAYD LLDI EQDT YRYE TTGL SEAR EKAV LLDE
Gene ID:	6813
Uniprot:	Q15833
Cellular Location:
Calculated MW:	66kDa/67kDa
Observed MW:	66kDa

Synonyms:

STXBP2, FHL5, Hunc18b, MUNC18-2, UNC18-2, UNC18B, pp10122

Background:

This gene encodes a member of the STXBP/unc-18/SEC1 family. The encoded protein is involved in intracellular trafficking, control of SNARE (soluble NSF attachment protein receptor) complex assembly, and the release of cytotoxic granules by natural killer cells. Mutations in this gene are associated with familial hemophagocytic lymphohistiocytosis. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

UniProt Protein Function:	STXBP2: Involved in intracellular vesicle trafficking and vesicle fusion with membranes. Contributes to the granule exocytosis machinery through interaction with soluble N- ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins that regulate membrane fusion. Regulates cytotoxic granule exocytosis in natural killer (NK) cells. Defects in STXBP2 are the cause of familial hemophagocytic lymphohistiocytosis type 5 (FHL5). FHL5 is rare disorder characterized by immune dysregulation with hypercytokinemia, defective function of natural killer cell, and massive infiltration of several organs by activated lymphocytes and macrophages. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, and less frequently neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits and ataxia. Belongs to the STXBP/unc-18/SEC1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 19p13.3-p13.2 Cellular Component: SNARE complex; specific granule; azurophil granule; plasma membrane; cytosol Molecular Function:protein binding; syntaxin-3 binding Biological Process: regulation of mast cell degranulation; protein transport; leukocyte mediated cytotoxicity; neutrophil degranulation; vesicle docking during exocytosis Disease: Hemophagocytic Lymphohistiocytosis, Familial, 5
NCBI Summary:	This gene encodes a member of the STXBP/unc-18/SEC1 family. The encoded protein is involved in intracellular trafficking, control of SNARE (soluble NSF attachment protein receptor) complex assembly, and the release of cytotoxic granules by natural killer cells. Mutations in this gene are associated with familial hemophagocytic lymphohistiocytosis. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2013]
UniProt Code:	Q15833
NCBI GenInfo Identifier:	313104015
NCBI Gene ID:	6813
NCBI Accession:	Q15833.2
UniProt Secondary Accession:	Q15833,Q9BU65, B4E175, E7EQD5,
UniProt Related Accession:	Q15833
Molecular Weight:	593
NCBI Full Name:	Syntaxin-binding protein 2
NCBI Synonym Full Names:	syntaxin binding protein 2
NCBI Official Symbol:	STXBP2
NCBI Official Synonym Symbols:	FHL5; UNC18B; Hunc18b; UNC18-2; pp10122; MUNC18-2
NCBI Protein Information:	syntaxin-binding protein 2; unc-18B; protein unc-18 homolog B
UniProt Protein Name:	Syntaxin-binding protein 2
UniProt Synonym Protein Names:	Protein unc-18 homolog 2; Unc18-2; Protein unc-18 homolog B; Unc-18B
Protein Family:	Syntaxin-binding protein
UniProt Gene Name:	STXBP2
UniProt Entry Name:	STXB2_HUMAN

	Western blot analysis of extracts of various cell lines, using STXBP2 antibody (CAB12511) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of A549 cells using STXBP2 antibody (CAB12511). Blue: DAPI for nuclear staining.

Antibodies

Anti-STXBP2 Antibody (CAB7735)

STXBP2 Antibody (PACO12565)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)