Anti-SULT2B1 Antibody (CAB7736)

Antibody Name:	Anti-SULT2B1 Antibody
Antibody SKU:	CAB7736
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-365 of human SULT2B1 (NP_814444.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, HT-29, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-365 of human SULT2B1 (NP_814444.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDGP AEPQ IPGL WDTY EDDI SEIS QKLP GEYF RYKG VPFP VGLY SLES ISLA ENTQ DVRD DDIF IITY PKSG TTWM IEII CLIL KEGD PSWI RSVP IWER APWC ETIV GAFS LPDQ YSPR LMSS HLPI QIFT KAFF SSKA KVIY MGRN PRDV VVSL YHYS KIAG QLKD PGTP DQFL RDFL KGEV QFGS WFDH IKGW LRMK GKDN FLFI TYEE LQQD LQGS VERI CGFL GRPL GKEA LGSV VAHS TFSA MKAN TMSN YTLL PPSL LDHR RGAF LRKG VCGD WKNH FTVA QSEA FDRA YRKQ MRGM PTFP WDED PEED GSPD PEPS PEPE PKPS LEPN TSLE REPR PNSS PSPS PGQA SETP HPRP S
Gene ID:	6820
Uniprot:	O00204
Cellular Location:	Cytoplasm, Microsome, Nucleus
Calculated MW:	39kDa/41kDa
Observed MW:	41kDa

Synonyms:

SULT2B1, HSST2

Background:

Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene sulfates dehydroepiandrosterone but not 4-nitrophenol, a typical substrate for the phenol and estrogen sulfotransferase subfamilies. Two alternatively spliced variants that encode different isoforms have been described.

UniProt Protein Function:	SULT2B1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs and xenobiotic compounds. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Sulfates hydroxysteroids like DHEA. Isoform 1 preferentially sulfonates cholesterol, and isoform 2 avidly sulfonates pregnenolone but not cholesterol. Belongs to the sulfotransferase 1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transferase; Lipid Metabolism - androgen and estrogen; Energy Metabolism - sulfur; EC 2.8.2.2 Chromosomal Location of Human Ortholog: 19q13.3 Cellular Component: intracellular membrane-bound organelle; endoplasmic reticulum; cytoplasm; cytosol; nucleus Molecular Function:protein binding; alcohol sulfotransferase activity; steroid sulfotransferase activity Biological Process: steroid metabolic process; sulfate assimilation; xenobiotic metabolic process; 3'-phosphoadenosine 5'-phosphosulfate metabolic process
NCBI Summary:	Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene sulfates dehydroepiandrosterone but not 4-nitrophenol, a typical substrate for the phenol and estrogen sulfotransferase subfamilies. Two alternatively spliced variants that encode different isoforms have been described. [provided by RefSeq]
UniProt Code:	O00204
NCBI GenInfo Identifier:	31563386
NCBI Gene ID:	6820
NCBI Accession:	NP_814444.1
UniProt Secondary Accession:	O00204,O00205, O75814,
UniProt Related Accession:	O00204
Molecular Weight:	41,308 Da
NCBI Full Name:	sulfotransferase family cytosolic 2B member 1 isoform b
NCBI Synonym Full Names:	sulfotransferase family, cytosolic, 2B, member 1
NCBI Official Symbol:	SULT2B1
NCBI Official Synonym Symbols:	HSST2
NCBI Protein Information:	sulfotransferase family cytosolic 2B member 1; ST2B1; sulfotransferase 2B1; alcohol sulfotransferase; hydroxysteroid sulfotransferase 2
UniProt Protein Name:	Sulfotransferase family cytosolic 2B member 1
UniProt Synonym Protein Names:	Alcohol sulfotransferase; Hydroxysteroid sulfotransferase 2
Protein Family:	Sulfotransferase family cytosolic
UniProt Gene Name:	SULT2B1
UniProt Entry Name:	ST2B1_HUMAN

	Western blot analysis of extracts of various cell lines, using SULT2B1 antibody (CAB7736) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver cancer using SULT2B1 antibody (CAB7736) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human kidney cancer using SULT2B1 antibody (CAB7736) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using SULT2B1 antibody (CAB7736) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using SULT2B1 antibody (CAB7736) at dilution of 1:100 (40x lens).

Antibodies

SULT2B1 Antibody (PACO12582)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)