Anti-SYCE1 Antibody (CAB7218)

Antibody Name:	Anti-SYCE1 Antibody
Antibody SKU:	CAB7218
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 132-351 of human SYCE1 (NP_001137236.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 132-351 of human SYCE1 (NP_001137236.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ECKE RISA LNLQ IEEE KNKQ RQLR LAFE EQLE DLMG QHKD LWDF HMPE RLAK EICA LDSS KEQL LKEE KLVK ATLE DVKH QLCS LCGA EGPS TLDE GLFL RSQE AAAT VQLF QEEH RKAE ELLA AAAQ RHQQ LQQK CQQQ QQKR QRLK EELE KHGM QVPA QAQS TQEE EAGP GDVA SPKP LKGE RPGA AHQA GPDV LIGQ EDTL HPDL SPRG FQEI KELF
Gene ID:	93426
Uniprot:	Q8N0S2
Cellular Location:	Chromosome, Nucleus
Calculated MW:	21kDa/32kDa/39kDa
Observed MW:	40kDa

Synonyms:

SYCE1, C10orf94, CT76, POF12, SPGF15

Background:

This gene encodes a member of the synaptonemal complex, which links homologous chromosomes during prophase I of meiosis. The tripartite structure of the complex is highly conserved amongst metazoans. It consists of two lateral elements and a central region formed by transverse elements and a central element. The protein encoded by this gene localizes to the central element and is required for initiation and elongation of the synapsis. Allelic variants of this gene have been associated with premature ovarian failure and spermatogenic failure. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	SYCE1: Major component of the transverse central element of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Requires SYCP1 in order to be incorporated into the central element. May have a role in the synaptonemal complex assembly, stabilization and recombination. Belongs to the SYCE family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cancer Testis Antigen (CTA) Chromosomal Location of Human Ortholog: 10q26.3 Cellular Component: central element Biological Process: synaptonemal complex assembly; cell division
UniProt Code:	Q8N0S2
NCBI GenInfo Identifier:	118573896
NCBI Gene ID:	93426
NCBI Accession:	Q8N0S2.2
UniProt Secondary Accession:	Q8N0S2,Q9D495, Q5XHZ2,
UniProt Related Accession:	Q8N0S2
Molecular Weight:	351
NCBI Full Name:	Synaptonemal complex central element protein 1
NCBI Synonym Full Names:	synaptonemal complex central element protein 1
NCBI Official Symbol:	SYCE1
NCBI Official Synonym Symbols:	CT76; C10orf94
NCBI Protein Information:	synaptonemal complex central element protein 1; cancer/testis antigen 76
UniProt Protein Name:	Synaptonemal complex central element protein 1
UniProt Synonym Protein Names:	Cancer/testis antigen 76; CT76
Protein Family:	Synaptonemal complex central element protein
UniProt Gene Name:	SYCE1
UniProt Entry Name:	SYCE1_HUMAN

	Western blot analysis of extracts of various cell lines, using SYCE1 antibody (CAB7218) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of MCF-7 cells using SYCE1 antibody (CAB7218). Blue: DAPI for nuclear staining.

Antibodies

SYCE1 Antibody (PACO12595)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)