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Anti-Syntaxin-12 Antibody (CAB8200)

SKU:
CAB8200
Product Type:
Antibody
Applications:
WB
IF
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • Cell Biology Antibodies 12 Anti-Syntaxin-12 Antibody CAB8200
  • Cell Biology Antibodies 12 Anti-Syntaxin-12 Antibody CAB8200
€139 - €419
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Description

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Antibody Name:STX12 Rabbit Polyclonal Antibody
Antibody SKU:CAB8200
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human STX12 (NP_803173.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse kidney, Mouse brain, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human STX12 (NP_803173.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSYG PLDM YRNP GPSG PQLR DFSS IIQT CSGN IQRI SQAT AQIK NLMS QLGT KQDS SKLQ ENLQ QLQH STNQ LAKE TNEL LKEL GSLP LPLS TSEQ RQQR LQKE RLMN DFSA ALNN FQAV QRRV SEKE KESI ARAR AGSR LSAE ERQR EEQL VSFD SHEE WNQM QSQE DEVA ITEQ DLEL IKER ETAI RQLE ADIL DVNQ IFKD LAMM IHDQ GDLI DSIE ANVE SSEV HVER ATEQ LQRA AYYQ KKSR KK
Gene ID:23673
Uniprot:Q86Y82
Cellular Location:Cytoplasmic side, Endomembrane system, Endosome membrane, Golgi apparatus membrane, Single-pass type IV membrane protein
Calculated MW:31kDa
Observed MW:31kDa
Synonyms:STX12, STX13, STX14
Background:
UniProt Protein Function:STX12: SNARE that acts to regulate protein transport between late endosomes and the trans-Golgi network. The SNARE complex containing STX6, STX12, VAMP4 and VTI1A mediates vesicle fusion (in vitro). Belongs to the syntaxin family.
UniProt Protein Details:

Protein type:Membrane protein, integral; Vesicle

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: endomembrane system; integral to membrane; lipid raft; phagocytic vesicle; SNARE complex; vesicle

Molecular Function:protein binding; SNAP receptor activity; SNARE binding

Biological Process: autophagic vacuole formation; cholesterol efflux; intracellular protein transport; protein stabilization; vesicle docking; vesicle fusion

UniProt Code:Q86Y82
NCBI GenInfo Identifier:47117211
NCBI Gene ID:23673
NCBI Accession:Q86Y82.1
UniProt Secondary Accession:Q86Y82,O95564, B1AJQ7,
UniProt Related Accession:Q86Y82
Molecular Weight:31,642 Da
NCBI Full Name:Syntaxin-12
NCBI Synonym Full Names:syntaxin 12
NCBI Official Symbol:STX12  
NCBI Official Synonym Symbols:STX13; STX14  
NCBI Protein Information:syntaxin-12
UniProt Protein Name:Syntaxin-12
Protein Family:Syntaxin
UniProt Gene Name:STX12  
UniProt Entry Name:STX12_HUMAN
Anti-Syntaxin-12 Polyclonal Antibody (CAB8200)Western blot analysis of extracts of various cell lines, using STX12 antibody (CAB8200) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-Syntaxin-12 Polyclonal Antibody (CAB8200)Immunofluorescence analysis of U2OS cells using STX12 antibody (CAB8200) at dilution of 1:100. Blue: DAPI for nuclear staining.

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