The STX12 Antibody (CAB8200) is a high-quality antibody developed for reliable detection and analysis of target proteins. Syntaxin 12 is a member of the SNARE protein family that plays a key role in membrane fusion events within the endosomal system. This antibody, raised in rabbits, specifically targets Syntaxin 12 and is highly reactive with human samples.Validated for use in Western blot applications, this antibody allows for the detection and analysis of Syntaxin 12 in various cell types. Its specificity and high reactivity make it an ideal choice for studies in cell biology, molecular biology, and neuroscience.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
STX12 Antibody
SKU:
CAB8200
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
STX13, STX14, STX12
Positive Sample:
Mouse kidney, Mouse brain, Rat kidney
Cellular Localization:
Cytoplasmic Side, Endomembrane System, Endosome Membrane, Golgi Apparatus Membrane, Single-Pass Type Iv Membrane Protein.
Calculated MW:
32kDa
Observed MW:
31kDa
Predicted to enable SNAP receptor activity and SNARE binding activity. Involved in autophagosome assembly; cholesterol efflux; and protein stabilization. Located in several cellular components, including membrane raft; phagocytic vesicle; and phagophore assembly site. Part of SNARE complex. Colocalizes with BLOC-1 complex.
Purification Method
Affinity purification
Gene ID
23673
RRID
AB_2772447
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using STX12 Rabbit pAb (CAB8200) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
