Anti-TBXAS1 Antibody (CAB13362)

Antibody Name:	Anti-TBXAS1 Antibody
Antibody SKU:	CAB13362
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 355-534 of human TBXAS1 (NP_001052.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	THP-1, A-549, NCI-H460

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 355-534 of human TBXAS1 (NP_001052.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TYLL ATNP DCQE KLLR EVDV FKEK HMAP EFCS LEEG LPYL DMVI AETL RMYP PAFR FTRE AAQD CEVL GQRI PAGA VLEM AVGA LHHD PEHW PSPE TFNP ERFT AEAR QQHR PFTY LPFG AGPR SCLG VRLG LLEV KLTL LHVL HKFR FQAC PETQ VPLQ LESK SALG PKNG VYIK IVSR
Gene ID:	6916
Uniprot:	P24557
Cellular Location:	Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:	52kDa/60kDa/65kDa
Observed MW:	50-56kDa

Synonyms:

TBXAS1, BDPLT14, CYP5, CYP5A1, GHOSAL, THAS, TS, TXAS, TXS

Background:

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to thromboxane A2, a potent vasoconstrictor and inducer of platelet aggregation. The enzyme plays a role in several pathophysiological processes including hemostasis, cardiovascular disease, and stroke. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	TBXAS1: Defects in TBXAS1 are the cause of Ghosal hematodiaphyseal dysplasia (GHDD). GHDD is a rare autosomal recessive disorder characterized by increased bone density with predominant diaphyseal involvement and aregenerative corticosteroid-sensitive anemia. Aregenerative anemia is characterized by bone marrow failure, so that functional marrow cells are regenerated slowly or not at all. Defects in TBXAS1 are the cause of thromboxane synthetase deficiency (TBXAS1 deficiency). It is characterized by hemorrhagic diathesis. Belongs to the cytochrome P450 family.
UniProt Protein Details:	Protein type:Oxidoreductase; Lipid Metabolism - arachidonic acid; Membrane protein, integral; Membrane protein, multi-pass; Isomerase; EC 5.3.99.5 Chromosomal Location of Human Ortholog: 7q34-q35 Cellular Component: endoplasmic reticulum membrane Molecular Function:thromboxane-A synthase activity Biological Process: cyclooxygenase pathway; icosanoid metabolic process Disease: Bleeding Disorder, Platelet-type, 14; Ghosal Hematodiaphyseal Dysplasia
NCBI Summary:	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to thromboxane A2, a potent vasoconstrictor and inducer of platelet aggregation. The enzyme plays a role in several pathophysiological processes including hemostasis, cardiovascular disease, and stroke. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
UniProt Code:	P24557
NCBI GenInfo Identifier:	254763392
NCBI Gene ID:	6916
NCBI Accession:	P24557.3
UniProt Secondary Accession:	P24557,O14987, Q16843, Q16844, Q8IUN1, Q96CN2, Q9GZW4 Q9HD77, B4DJG6, E7EMU9, E7EP08, E7ESB5,
UniProt Related Accession:	P24557
Molecular Weight:	52,287 Da
NCBI Full Name:	Thromboxane-A synthase
NCBI Synonym Full Names:	thromboxane A synthase 1
NCBI Official Symbol:	TBXAS1
NCBI Official Synonym Symbols:	TS; TXS; CYP5; THAS; TXAS; CYP5A1; GHOSAL; BDPLT14
NCBI Protein Information:	thromboxane-A synthase
UniProt Protein Name:	Thromboxane-A synthase
UniProt Synonym Protein Names:	Cytochrome P450 5A1
Protein Family:	Thromboxane-A synthase
UniProt Gene Name:	TBXAS1
UniProt Entry Name:	THAS_HUMAN

	Western blot analysis of extracts of various cell lines, using TBXAS1 antibody (CAB13362) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded mouse heart using TBXAS1 antibody (CAB13362) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using TBXAS1 antibody (CAB13362). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-TBXAS1 Antibody (CAB1988)	Human Thromboxane-A synthase / TBXAS1 ELISA Kit
Anti-Thromboxane synthase Antibody (CAB5173)	Human Thromboxane-A synthase (TBXAS1) ELISA Kit
TBXAS1 Antibody (PACO05176)
TBXAS1 Antibody (PACO12664)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)