Anti-TCAP Antibody (CAB13566)

Antibody Name:	Anti-TCAP Antibody
Antibody SKU:	CAB13566
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-167 of human TCAP (NP_003664.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse heart, Mouse skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-167 of human TCAP (NP_003664.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MATS ELSC EVSE ENCE RREA FWAE WKDL TLST RPEE GCSL HEED TQRH ETYH QQGQ CQVL VQRS PWLM MRMG ILGR GLQE YQLP YQRV LPLP IFTP AKMG ATKE ERED TPIQ LQEL LALE TALG GQCV DRQE VAEI TKQL PPVV PVSK PGAL RRSL SRSM SQEA QRG
Gene ID:	8557
Uniprot:	O15273
Cellular Location:	Cytoplasm, myofibril, sarcomere
Calculated MW:	19kDa
Observed MW:	19kDa

Synonyms:

TCAP, CMD1N, CMH25, LGMD2G, T-cap, TELE, telethonin, titin-cap

Background:

Sarcomere assembly is regulated by the muscle protein titin. Titin is a giant elastic protein with kinase activity that extends half the length of a sarcomere. It serves as a scaffold to which myofibrils and other muscle related proteins are attached. This gene encodes a protein found in striated and cardiac muscle that binds to the titin Z1-Z2 domains and is a substrate of titin kinase, interactions thought to be critical to sarcomere assembly. Mutations in this gene are associated with limb-girdle muscular dystrophy type 2G.

UniProt Protein Function:	Telethonin: a muscle assembly regulating factor that interacts with the giant kinase titin. Sarcomere assembly is regulated by the muscle protein titin. Titin is a giant elastic protein with kinase activity that extends half the length of a sarcomere. It serves as a scaffold to which myofibrils and other muscle related proteins are attached. Telethonin is found in striated and cardiac muscle that binds to the titin Z1-Z2 domains and is a substrate of titin kinase, interactions thought to be critical to sarcomere assembly. Mutations in this gene are associated with limb-girdle muscular dystrophy type 2G.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 17q12 Cellular Component: I band; cytosol; Z disc Molecular Function:protein binding, bridging; protein binding; structural constituent of muscle; titin binding; FATZ binding Biological Process: adult heart development; skeletal muscle contraction; cardiac muscle hypertrophy; somitogenesis; muscle thin filament assembly; detection of mechanical stimulus; cardiac myofibril assembly; sarcomere organization; muscle filament sliding; muscle thick filament assembly; cardiac muscle fiber development; cardiac muscle morphogensis; sarcomerogenesis; protein complex assembly; otic vesicle formation; cardiac muscle contraction Disease: Muscular Dystrophy, Limb-girdle, Type 2g; Cardiomyopathy, Dilated, 1n
NCBI Summary:	Sarcomere assembly is regulated by the muscle protein titin. Titin is a giant elastic protein with kinase activity that extends half the length of a sarcomere. It serves as a scaffold to which myofibrils and other muscle related proteins are attached. This gene encodes a protein found in striated and cardiac muscle that binds to the titin Z1-Z2 domains and is a substrate of titin kinase, interactions thought to be critical to sarcomere assembly. Mutations in this gene are associated with limb-girdle muscular dystrophy type 2G. [provided by RefSeq, Jul 2008]
UniProt Code:	O15273
NCBI GenInfo Identifier:	3024716
NCBI Gene ID:	8557
NCBI Accession:	O15273.1
UniProt Secondary Accession:	O15273,Q96L27,
UniProt Related Accession:	O15273
Molecular Weight:	Observed MW: 19kDaCalculated MW: 19kDa
NCBI Full Name:	Telethonin
NCBI Synonym Full Names:	titin-cap
NCBI Official Symbol:	TCAP
NCBI Official Synonym Symbols:	TELE; CMD1N; T-cap; LGMD2G; telethonin
NCBI Protein Information:	telethonin; titin cap protein; 19 kDa sarcomeric protein; limb girdle muscular dystrophy 2G (autosomal recessive)
UniProt Protein Name:	Telethonin
UniProt Synonym Protein Names:	Titin cap protein
Protein Family:	Telethonin
UniProt Gene Name:	TCAP
UniProt Entry Name:	TELT_HUMAN

	Western blot analysis of extracts of various cell lines, using TCAP antibody (CAB13566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of HeLa cells using TCAP antibody (CAB13566). Blue: DAPI for nuclear staining.

Antibodies

Anti-TCAP Antibody (CAB6765)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)