null

Anti-TCF1/TCF7 Antibody (CAB20835)

SKU:
CAB20835
Product Type:
Antibody
Antibody Type:
Secondary Antibody
Host Species:
Mouse
Isotype:
IgG
  • Immunohistochemistry of paraffin-embedded human tonsil using TCF1/TCF7 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver cancer using TCF1/TCF7 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
  • Western blot analysis of extracts of Rat lung, using TCF1/TCF7 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using TCF1/TCF7 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
  • Western blot analysis of extracts of various cell lines, using TCF1/TCF7 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
€179 - €469
Frequently bought together:

Description

Antibody Name:Anti-TCF1/TCF7 Antibody (CAB20835)
Antibody SKU:CAB20835
Antibody Size:50µL, 100µL
Application:Western blotting, Immunohistochemistry
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 50-150 of human TCF1/TCF7 (NP_003193.2).
Application:Western blotting, Immunohistochemistry
Recommended Dilution:WB 1:500 - 1:1000
IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, MOLT-4, Mouse thymus, Rat lung
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 50-150 of human TCF1/TCF7 (NP_003193.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ELKS SLVN ESEG AAGG AGIP GVPG AGAG ARGE AEAL GREH AAQR LFPD KLPE PLED GLKA PECT SGMY KETV YSAF NLLM HYPP PSGA GQHP QPQP PLHK A
Cellular Location:Nucleus
Calculated MW:28-42kDa/53-54kDa
Observed MW:28-50KDa
Synonyms:TCF7, TCF-1
Background:The protein encoded by this gene is a transcriptional activator that plays an important role in lymphocyte differentiation. This gene is expressed predominantly in T-cells. The encoded protein can bind an enhancer element and activate the CD3E gene, and it also may repress the CTNNB1 and TCF7L2 genes through a feedback mechanism. Several transcript variants encoding different isoforms have been found for this gene.
Immunohistochemistry of paraffin-embedded human tonsil using TCF1/TCF7 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded human tonsil using TCF1/TCF7 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human liver cancer using TCF1/TCF7 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded human liver cancer using TCF1/TCF7 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Western blot analysis of extracts of Rat lung, using TCF1/TCF7 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.Western blot analysis of extracts of Rat lung, using TCF1/TCF7 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using TCF1/TCF7 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.Immunohistochemistry of paraffin-embedded human colon carcinoma using TCF1/TCF7 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
Western blot analysis of extracts of various cell lines, using TCF1/TCF7 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Western blot analysis of extracts of various cell lines, using TCF1/TCF7 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Related Products