Anti-TCP1 Antibody (CAB1950)

Antibody Name:	Anti-TCP1 Antibody
Antibody SKU:	CAB1950
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 307-556 of human TCP1 (NP_110379.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	293T, PC-3, HeLa, Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 307-556 of human TCP1 (NP_110379.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AVRR VLKR DLKR IAKA SGAT ILST LANL EGEE TFEA AMLG QAEE VVQE RICD DELI LIKN TKAR TSAS IILR GAND FMCD EMER SLHD ALCV VKRV LESK SVVP GGGA VEAA LSIY LENY ATSM GSRE QLAI AEFA RSLL VIPN TLAV NAAQ DSTD LVAK LRAF HNEA QVNP ERKN LKWI GLDL SNGK PRDN KQAG VFEP TIVK VKSL KFAT EAAI TILR IDDL IKLH PESK DDKH GSYE DAVH SGAL ND
Gene ID:	6950
Uniprot:	P17987
Cellular Location:	Cytoplasm, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:	60kDa
Observed MW:	60kDa

Synonyms:

TCP1, CCT-alpha, CCT1, CCTa, D6S230E, TCP-1-alpha

Background:

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found.

UniProt Protein Function:	CCT-alpha: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG. Component of the BBS/CCT complex composed at least of MKKS, BBS10, BBS12, TCP1, CCT2, CCT3, CCT4, CCT5 AND CCT8. Belongs to the TCP-1 chaperonin family.
UniProt Protein Details:	Protein type:Chaperone; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 6q25.3-q26 Cellular Component: chaperonin-containing T-complex; Golgi apparatus; centrosome; microtubule; pericentriolar material; acrosome; nuclear heterochromatin; cytosol Molecular Function:protein binding; ubiquitin protein ligase binding; unfolded protein binding; ATP binding Biological Process: tubulin folding; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; binding of sperm to zona pellucida
NCBI Summary:	The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found. [provided by RefSeq, Jun 2010]
UniProt Code:	P17987
NCBI GenInfo Identifier:	135538
NCBI Gene ID:	6950
NCBI Accession:	P17987.1
UniProt Secondary Accession:	P17987,Q15556, Q5TCM3, E1P5B2,
UniProt Related Accession:	P17987
Molecular Weight:	556
NCBI Full Name:	T-complex protein 1 subunit alpha
NCBI Synonym Full Names:	t-complex 1
NCBI Official Symbol:	TCP1
NCBI Official Synonym Symbols:	CCT1; CCTa; D6S230E; CCT-alpha; TCP-1-alpha
NCBI Protein Information:	T-complex protein 1 subunit alpha; tailless complex polypeptide 1; T-complex protein 1, alpha subunit
UniProt Protein Name:	T-complex protein 1 subunit alpha
UniProt Synonym Protein Names:	CCT-alpha
Protein Family:	T-complex protein
UniProt Gene Name:	TCP1
UniProt Entry Name:	TCPA_HUMAN

	Western blot analysis of extracts of various cell lines, using TCP1 antibody (CAB1950) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat kidney using TCP1 antibody (CAB1950) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using TCP1 antibody (CAB1950). Blue: DAPI for nuclear staining.

Antibodies

Anti-TCP1 Antibody (CAB13364)

TCP1 Antibody (PACO05182)

TCP1 Antibody (PACO12679)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)