Western blot analysis of various lysates, using TEAD4 Rabbit mAb (CAB23774) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The TEAD4 Monoclonal Antibody (CAB23774) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using cutting-edge technology, specifically targets TEAD4 in human samples and is validated for use in immunofluorescence and immunohistochemistry applications.TEAD4, a key player in the Hippo signaling pathway, regulates gene expression and influences cell fate decisions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
TEAD4 Monoclonal Antibody
SKU:
CAB23774
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC59949
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
SATA FHSS MALA RGPG RPAV SGFW QGAL PGQA GTSH DVKP FSQQ TYAV QPPL PLPG FESP AGPA PSPS APPA PPWQ GRSV ASSK LWML EFSA FLEQ QQDP D
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression. Alternatively spliced transcripts encoding distinct isoforms, some of which are translated through the use of a non-AUG (UUG) initiation codon, have been described for this gene.
Purification Method
Affinity purification
Gene ID
7004
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using TEAD4 Rabbit mAb (CAB23774) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
