Anti-TES Antibody (CAB7094)

Antibody Name:	Anti-TES Antibody
Antibody SKU:	CAB7094
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human TES (NP_056456.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	HeLa, 22Rv1, Rat ovary

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human TES (NP_056456.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDLE NKVK KMGL GHEQ GFGA PCLK CKEK CEGF ELHF WRKI CRNC KCGQ EEHD VLLS NEED RKVG KLFE DTKY TTLI AKLK SDGI PMYK RNVM ILTN PVAA KKNV SINT VTYE WAPP VQNQ ALAR QYMQ MLPK EKQP VAGS EGAQ YRKK QLAK QLPA HDQD PSKC HELS PREV KEME QFVK KYKS EALG VGDV KLPC EMDA QGPK QMNI PGGD RSTP AAVG AMED KSAE HKRT QYSC YCCK LSMK EGDP AIYA ERAG YDKL
Gene ID:	26136
Uniprot:	Q9UGI8
Cellular Location:	Cell junction, Cytoplasm, focal adhesion
Calculated MW:	46kDa/47kDa
Observed MW:	47kDa

Synonyms:

TES, TESS, TESS-2, testin

Background:

Cancer-associated chromosomal changes often involve regions containing fragile sites. This gene maps to a commom fragile site on chromosome 7q31.2 designated FRA7G. This gene is similar to mouse Testin, a testosterone-responsive gene encoding a Sertoli cell secretory protein containing three LIM domains. LIM domains are double zinc-finger motifs that mediate protein-protein interactions between transcription factors, cytoskeletal proteins and signaling proteins. This protein is a negative regulator of cell growth and may act as a tumor suppressor. This scaffold protein may also play a role in cell adhesion, cell spreading and in the reorganization of the actin cytoskeleton. Multiple protein isoforms are encoded by transcript variants of this gene.

UniProt Protein Function:	TES: Scaffold protein that may play a role in cell adhesion, cell spreading and in the reorganization of the actin cytoskeleton. Plays a role in the regulation of cell proliferation. May act as a tumor suppressor. Inhibits tumor cell growth. Belongs to the prickle / espinas / testin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Cytoskeletal Chromosomal Location of Human Ortholog: 7q31.2 Cellular Component: protein complex; focal adhesion; cytoplasm; plasma membrane; nucleus; cell junction Molecular Function:zinc ion binding Biological Process: negative regulation of cell proliferation
UniProt Code:	Q9UGI8
NCBI GenInfo Identifier:	17380320
NCBI Gene ID:
NCBI Accession:	Q9UGI8.1
Molecular Weight:	46kDa; 47kDa
NCBI Full Name:	Testin
UniProt Protein Name:	Testin
UniProt Synonym Protein Names:	TESS
Protein Family:	Tesmin
UniProt Gene Name:	TES
UniProt Entry Name:	TES_HUMAN

	Western blot analysis of extracts of various cell lines, using TES antibody (CAB7094) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat ovary using TES antibody (CAB7094) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using TES antibody (CAB7094) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver cancer using TES antibody (CAB7094) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human placenta using TES antibody (CAB7094) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
TES Antibody (PACO12698)	Human Testin ELISA Kit
	Human Testin (TES) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)