Anti-TFAM Antibody (CAB13552)

Antibody Name:	Anti-TFAM Antibody
Antibody SKU:	CAB13552
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TFAM (NP_003192.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	SW620, HT-29

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TFAM (NP_003192.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAFL RSMW GVLS ALGR SGAE LCTG CGSR LRSP FSFV YLPR WFSS VLAS CPKK PVSS YLRF SKEQ LPIF KAQN PDAK TTEL IRRI AQRW RELP DSKK KIYQ DAYR AEWQ VYKE EISR FKEQ LTPS QIMS LEKE IMDK HLKR KAMT KKKE LTLL GKPK RPRS AYNV YVAE RFQE AKGD SPQE KLKT VKEN WKNL SDSE KELY IQHA KEDE TRYH NEMK SWEE
Gene ID:	7019
Uniprot:	Q00059
Cellular Location:	Mitochondrion, Mitochondrion matrix, mitochondrion nucleoid
Calculated MW:	25kDa/29kDa
Observed MW:	24kDa

Synonyms:

TFAM, MTDPS15, MTTF1, MTTFA, TCF6, TCF6L1, TCF6L2, TCF6L3

Background:

This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	TFAM: regulates mitochondrial transcription. Induced in humans by caloric restriction. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Activates transcription by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Involved in cisplatin resistance. Interacts with TFB1M and TFB2M.
UniProt Protein Details:	Protein type:Transcription regulation; DNA-binding Chromosomal Location of Human Ortholog: 10q21 Cellular Component: mitochondrion; mitochondrial matrix; nucleus; cytosol Molecular Function:protein binding; heat shock protein binding; chromatin binding; transcription factor activity; DNA bending activity Biological Process: mitochondrial respiratory chain complex assembly; chromatin remodeling; mitochondrion organization and biogenesis; regulation of transcription from RNA polymerase I promoter; positive regulation of transcription, DNA-dependent; organelle organization and biogenesis; gene expression; positive regulation of transcription from RNA polymerase II promoter; DNA-dependent DNA replication; transcription from mitochondrial promoter; transcription initiation from mitochondrial promoter
NCBI Summary:	This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
UniProt Code:	Q00059
NCBI GenInfo Identifier:	417324
NCBI Gene ID:	7019
NCBI Accession:	Q00059.1
UniProt Secondary Accession:	Q00059,Q5U0C6, A8MRB2, A9QXC6, B5BU05,
UniProt Related Accession:	Q00059
Molecular Weight:	246
NCBI Full Name:	Transcription factor A, mitochondrial
NCBI Synonym Full Names:	transcription factor A, mitochondrial
NCBI Official Symbol:	TFAM
NCBI Official Synonym Symbols:	TCF6; MTTF1; MTTFA; TCF6L1; TCF6L2; TCF6L3
NCBI Protein Information:	transcription factor A, mitochondrial; transcription factor 6; transcription factor 6-like 1; transcription factor 6-like 3; mitochondrial transcription factor 1; mitochondrial transcription factor A; transcription factor 6-like 2 (mitochondrial transcription factor)
UniProt Protein Name:	Transcription factor A, mitochondrial
UniProt Synonym Protein Names:	Mitochondrial transcription factor 1; MtTF1; Transcription factor 6; TCF-6; Transcription factor 6-like 2
Protein Family:	Transcription factor
UniProt Gene Name:	TFAM
UniProt Entry Name:	TFAM_HUMAN

	Western blot analysis of extracts of various cell lines, using TFAM antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat brain using TFAM antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded human colon using TFAM antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with IHC staining protocol.
	Immunofluorescence analysis of NIH/3T3 cells using TFAM Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of PC-12 cells using TFAM Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Chromatin immunoprecipitation analysis of extracts of K-562 cells, using TFAM antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Antibodies

Anti-TFAM Antibody (CAB1926)

Anti-TFAM Antibody (CAB3173)

TFAM Antibody (PACO03106)

TFAM Antibody (PACO12707)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)