Anti-TGFBR2 Antibody (CAB1415)
- SKU:
- CAB1415
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
| Antibody Name: | Anti-TGFBR2 Antibody |
| Antibody SKU: | CAB1415 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-280 of human TGFBR2 (NP_003233.4). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-29, A-549, Mouse lung, Mouse spleen, Mouse brain, Mouse kidney, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-280 of human TGFBR2 (NP_003233.4). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TIPP HVQK SVNN DMIV TDNN GAVK FPQL CKFC DVRF STCD NQKS CMSN CSIT SICE KPQE VCVA VWRK NDEN ITLE TVCH DPKL PYHD FILE DAAS PKCI MKEK KKPG ETFF MCSC SSDE CNDN IIFS EEYN TSNP DLLL VIFQ VTGI SLLP PLGV AISV IIIF YCYR VNRQ QKLS STWE TGKT RKLM EFSE HCAI ILED DRSD ISST CANN INHN TELL PIEL DTLV GKGR FAEV YKAK LKQN TSEQ FETV AVKI FP |
| Gene ID: | 7048 |
| Uniprot: | P37173 |
| Cellular Location: | Cell membrane, Single-pass type I membrane protein |
| Calculated MW: | 64kDa/67kDa |
| Observed MW: | 75kDa |
| Synonyms: | AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TGFR-2, TGFbeta-RII, TGF beta Receptor II, TGFBR2 |
| Background: | This gene encodes a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. The encoded protein is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in this gene have been associated with Marfan Syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different isoforms have been characterized. |
| UniProt Protein Function: | TGFBR2: a TKL kinase of the serine/threonine-protein kinase receptor (STKR) family. R1 and R2 TGF-beta receptors dimerize after binding TGF-beta at the cell surface. Binds to DAXX. Defects can cause esophageal cancer. |
| UniProt Protein Details: | Protein type:Oncoprotein; Membrane protein, integral; Protein kinase, TKL; EC 2.7.11.30; Protein kinase, Ser/Thr (receptor); Kinase, protein; TKL group; STKR family; Type2 subfamily Chromosomal Location of Human Ortholog: 3p22 Cellular Component: caveola; cytosol; external side of plasma membrane; integral to membrane; lipid raft; plasma membrane; receptor complex Molecular Function:ATP binding; glycosaminoglycan binding; metal ion binding; mitogen-activated protein kinase kinase kinase binding; protein binding; receptor signaling protein serine/threonine kinase activity; SMAD binding; transforming growth factor beta binding; transforming growth factor beta receptor activity; transforming growth factor beta receptor activity, type II; transmembrane receptor protein serine/threonine kinase activity Biological Process: activation of protein kinase activity; aging; apoptosis; blood vessel development; brain development; common-partner SMAD protein phosphorylation; embryo implantation; embryonic cranial skeleton morphogenesis; embryonic hemopoiesis; gastrulation; gut development; heart development; in utero embryonic development; lens development in camera-type eye; myeloid dendritic cell differentiation; negative regulation of cardiac muscle cell proliferation; negative regulation of transforming growth factor beta receptor signaling pathway; Notch signaling pathway; organ regeneration; palate development; patterning of blood vessels; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of angiogenesis; positive regulation of B cell tolerance induction; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of NK T cell differentiation; positive regulation of skeletal muscle regeneration; positive regulation of smooth muscle cell proliferation; positive regulation of T cell tolerance induction; positive regulation of tolerance induction to self antigen; protein amino acid phosphorylation; receptor-mediated endocytosis; regulation of cell proliferation; regulation of gene expression; response to drug; response to estrogen stimulus; response to glucose stimulus; response to mechanical stimulus; response to nutrient; smoothened signaling pathway; transforming growth factor beta receptor signaling pathway; vasculogenesis; wound healing Disease: Colorectal Cancer, Hereditary Nonpolyposis, Type 6; Esophageal Cancer; Loeys-dietz Syndrome 2 |
| NCBI Summary: | This gene encodes a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. The encoded protein is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in this gene have been associated with Marfan Syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different isoforms have been characterized. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P37173 |
| NCBI GenInfo Identifier: | 116242818 |
| NCBI Gene ID: | 7048 |
| NCBI Accession: | P37173.2 |
| UniProt Secondary Accession: | P37173,Q15580, Q6DKT6, Q99474, B4DTV5, |
| UniProt Related Accession: | P37173 |
| Molecular Weight: | 67,457 Da |
| NCBI Full Name: | TGF-beta receptor type-2 |
| NCBI Synonym Full Names: | transforming growth factor beta receptor II |
| NCBI Official Symbol: | TGFBR2Â Â |
| NCBI Official Synonym Symbols: | AAT3; FAA3; LDS2; MFS2; RIIC; LDS1B; LDS2B; TAAD2; TGFR-2; TGFbeta-RIIÂ Â |
| NCBI Protein Information: | TGF-beta receptor type-2 |
| UniProt Protein Name: | TGF-beta receptor type-2 |
| UniProt Synonym Protein Names: | TGF-beta type II receptor; Transforming growth factor-beta receptor type II; TGF-beta receptor type II; TbetaR-II |
| Protein Family: | TGF-beta receptor |
| UniProt Gene Name: | TGFBR2Â Â |
| UniProt Entry Name: | TGFR2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TGFBR2 antibody (CAB1415) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Immunohistochemistry of paraffin-embedded human appendix using TGFBR2 antibody (CAB1415) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast cancer using TGFBR2 antibody (CAB1415) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of NIH/3T3 cells using TGFBR2 antibody (CAB1415) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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