Anti-TGM1 Antibody (CAB13359)
- SKU:
- CAB13359
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-TGM1 Antibody |
| Antibody SKU: | CAB13359 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-280 of human TGM1 (NP_000350.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, Mouse kidney, Rat kidney, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 100-280 of human TGM1 (NP_000350.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AGDG TIRE GMLV VNGV DLLS SRSD QNRR EHHT DEYE YDEL IVRR GQPF HMLL LLSR TYES SDRI TLEL LIGN NPEV GKGT HVII PVGK GGSG GWKA QVVK ASGQ NLNL RVHT SPNA IIGK FQFT VRTQ SDAG EFQL PFDP RNEI YILF NPWC PEDI VYVD HEDW RQEY VLNE SGRI YYGT E |
| Gene ID: | 7051 |
| Uniprot: | P22735 |
| Cellular Location: | Lipid-anchor, Membrane |
| Calculated MW: | 40kDa/89kDa |
| Observed MW: | 90kDa |
| Synonyms: | TGM1, ARCI1, ICR2, KTG, LI, LI1, TGASE, TGK |
| Background: | The protein encoded by this gene is a membrane protein that catalyzes the addition of an alkyl group from an akylamine to a glutamine residue of a protein, forming an alkylglutamine in the protein. This protein alkylation leads to crosslinking of proteins and catenation of polyamines to proteins. This gene contains either one or two copies of a 22 nt repeat unit in its 3' UTR. Mutations in this gene have been associated with autosomal recessive lamellar ichthyosis (LI) and nonbullous congenital ichthyosiform erythroderma (NCIE). |
| UniProt Protein Function: | TGM1: an enzyme of the transglutaminase family that catalyzes the crosslinking of proteins and the conjugation of polyamines to proteins. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. Responsible for cross-linking epidermal proteins during formation of the stratum corneum. |
| UniProt Protein Details: | Protein type:Transferase; EC 2.3.2.13 Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: cell-cell adherens junction; cornified envelope; intrinsic to membrane Molecular Function:protein binding; protein-glutamine gamma-glutamyltransferase activity; metal ion binding Biological Process: cell envelope organization and biogenesis; keratinocyte differentiation; organ morphogenesis; keratinization; protein modification process; peptide cross-linking Disease: Ichthyosis, Congenital, Autosomal Recessive 1 |
| NCBI Summary: | The protein encoded by this gene is a membrane protein that catalyzes the addition of an alkyl group from an akylamine to a glutamine residue of a protein, forming an alkylglutamine in the protein. This protein alkylation leads to crosslinking of proteins and catenation of polyamines to proteins. This gene contains either one or two copies of a 22 nt repeat unit in its 3' UTR. Mutations in this gene have been associated with autosomal recessive lamellar ichthyosis (LI) and nonbullous congenital ichthyosiform erythroderma (NCIE). [provided by RefSeq, Jul 2008] |
| UniProt Code: | P22735 |
| NCBI GenInfo Identifier: | 57015359 |
| NCBI Gene ID: | 7051 |
| NCBI Accession: | P22735.4 |
| UniProt Related Accession: | P22735 |
| Molecular Weight: | Observed: 90 kDaPredicted: 90 kDa |
| NCBI Full Name: | Protein-glutamine gamma-glutamyltransferase K |
| NCBI Synonym Full Names: | transglutaminase 1 |
| NCBI Official Symbol: | TGM1Â Â |
| NCBI Official Synonym Symbols: | LI; KTG; LI1; TGK; ICR2; ARCI1; TGASEÂ Â |
| NCBI Protein Information: | protein-glutamine gamma-glutamyltransferase K |
| UniProt Protein Name: | Protein-glutamine gamma-glutamyltransferase K |
| UniProt Synonym Protein Names: | Epidermal TGase; Transglutaminase K; TG(K); TGK; TGase K; Transglutaminase-1; TGase-1 |
| Protein Family: | Protein-glutamine gamma-glutamyltransferase |
| UniProt Gene Name: | TGM1Â Â |
| UniProt Entry Name: | TGM1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TGM1 antibody (CAB13359) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunofluorescence analysis of HeLa cells using TGM1 antibody (CAB13359) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of rat skin cells using TGM1 antibody (CAB13359) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of mouse skin cells using TGM1 antibody (CAB13359) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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