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Anti-THPO Antibody (CAB1825)

SKU:
CAB1825
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-THPO Antibody
Antibody SKU:CAB1825
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 22-349 of human THPO (NP_001171068.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:MCF7, U-937, BT-474
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 22-349 of human THPO (NP_001171068.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SPAP PACD LRVL SKLL RDSH VLHS RLSQ CPEV HPLP TPVL LPAV DFSL GEWK TQME ETKA QDIL GAVT LLLE GVMA ARGQ LGPT CLSS LLGQ LSGQ VRLL LGAL QSLL GTQG RTTA HKDP NAIF LSFQ HLLR GKVR FLML VGGS TLCV RRAP PTTA VPSR TSLV LTLN ELPN RTSG LLET NFTA SART TGSG LLKW QQGF RAKI PGLL NQTS RSLD QIPG YLNR IHEL LNGT RGLF PGPS RRTL GAPD ISSG TSDT GSLP PNLQ PGYS PSPT HPPT GQYT LFPL PPTL PTPV VQLH PLLP DPSA PTPT PTSP LLNT SYTH SQNL SQEG
Gene ID:7066
Uniprot:P40225
Cellular Location:Secreted
Calculated MW:33kDa/37kDa
Observed MW:32kDa
Synonyms:THPO, MGDF, MKCSF, ML, MPLLG, THCYT1, TPO
Background:Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent.
UniProt Protein Function:THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell cycle regulation; Secreted; Cytokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular space

Molecular Function:growth factor activity; hormone activity; cytokine activity

Biological Process: positive regulation of protein kinase B signaling cascade; cell proliferation; platelet activation; multicellular organismal development; myeloid cell differentiation; positive regulation of megakaryocyte differentiation; positive regulation of protein amino acid phosphorylation; blood coagulation

Disease: Thrombocythemia 1

NCBI Summary:Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. [provided by RefSeq, Feb 2014]
UniProt Code:P40225
NCBI GenInfo Identifier:730982
NCBI Gene ID:7066
NCBI Accession:P40225.1
UniProt Related Accession:P40225
Molecular Weight:
NCBI Full Name:Thrombopoietin
NCBI Synonym Full Names:thrombopoietin
NCBI Official Symbol:THPO  
NCBI Official Synonym Symbols:ML; TPO; MGDF; MKCSF; MPLLG; THCYT1  
NCBI Protein Information:thrombopoietin
UniProt Protein Name:Thrombopoietin
UniProt Synonym Protein Names:C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand
Protein Family:Thyroid peroxidase
UniProt Gene Name:THPO  
UniProt Entry Name:TPO_HUMAN
Anti-THPO Antibody (CAB1825)Western blot analysis of extracts of various cell lines, using THPO antibody (CAB1825) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021).

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