Anti-THY1 Antibody (CAB2126)

Antibody Name:	Anti-THY1 Antibody
Antibody SKU:	CAB2126
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-130 of human THY1 (NP_006279.2).

Application:	WB IF
Recommended Dilution:	WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	Mouse brain, Mouse kidney, Mouse lung, Rat brain, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-130 of human THY1 (NP_006279.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QKVT SLTA CLVD QSLR LDCR HENT SSSP IQYE FSLT RETK KHVL FGTV GVPE HTYR SRTN FTSK YNMK VLYL SAFT SKDE GTYT CALH HSGH SPPI SSQN VTVL RDKL VKC
Gene ID:	7070
Uniprot:	P04216
Cellular Location:	Cell membrane, GPI-anchor, Lipid-anchor
Calculated MW:	17kDa
Observed MW:	25kDa

Synonyms:

THY1, CD90, CDw90

Background:

This gene encodes a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. The encoded protein is involved in cell adhesion and cell communication in numerous cell types, but particularly in cells of the immune and nervous systems. The encoded protein is widely used as a marker for hematopoietic stem cells. This gene may function as a tumor suppressor in nasopharyngeal carcinoma. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	THY1: May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
UniProt Protein Details:	Protein type:Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, GPI anchor Chromosomal Location of Human Ortholog: 11q23.3 Cellular Component: anchored to external side of plasma membrane; focal adhesion; growth cone; integral to plasma membrane; endoplasmic reticulum; dendrite; apical plasma membrane; plasma membrane; cytosol; lipid raft; external side of plasma membrane Molecular Function:integrin binding; protein binding; protein kinase binding Biological Process: focal adhesion formation; negative regulation of T cell receptor signaling pathway; cytoskeleton organization and biogenesis; T cell receptor signaling pathway; cell-cell adhesion; retinal cone cell development; negative regulation of protein kinase activity; negative regulation of axonogenesis; positive regulation of T cell activation; angiogenesis; positive regulation of release of sequestered calcium ion into cytosol; negative regulation of cell migration; positive regulation of GTPase activity
NCBI Summary:	This gene encodes a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. The encoded protein is involved in cell adhesion and cell communication in numerous cell types, but particularly in cells of the immune and nervous systems. The encoded protein is widely used as a marker for hematopoietic stem cells. This gene may function as a tumor suppressor in nasopharyngeal carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
UniProt Code:	P04216
NCBI GenInfo Identifier:	20455514
NCBI Gene ID:	7070
NCBI Accession:	P04216.2
UniProt Secondary Accession:	P04216,Q16008, Q9NSP1,
UniProt Related Accession:	P04216
Molecular Weight:	17,935 Da
NCBI Full Name:	Thy-1 membrane glycoprotein
NCBI Synonym Full Names:	Thy-1 cell surface antigen
NCBI Official Symbol:	THY1
NCBI Official Synonym Symbols:	CD90
NCBI Protein Information:	thy-1 membrane glycoprotein; CDw90; thy-1 antigen; Thy-1 T-cell antigen
UniProt Protein Name:	Thy-1 membrane glycoprotein
UniProt Synonym Protein Names:	CDw90; Thy-1 antigen; CD_antigen: CD90
Protein Family:	Thy-1 membrane glycoprotein
UniProt Gene Name:	THY1
UniProt Entry Name:	THY1_HUMAN

	Western blot analysis of extracts of various cell lines, using THY1 antibody (CAB2126) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of Mouse brain cells using THY1 antibody (CAB2126) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of Rat brain cells using THY1 antibody (CAB2126) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Human CD90 Monoclonal Antibody (FITC Conjugated) [5E10](AGEL1828)	Human Thy-1 membrane glycoprotein (THY1) ELISA Kit
Human CD90 Monoclonal Antibody (PE Conjugated) [5E10](AGEL1829)
Human CD90 Monoclonal Antibody (APC Conjugated) [5E10](AGEL1830)
Human CD90 Monoclonal Antibody (PE/Cyanine5 Conjugated) [5E10](AGEL1831)
Human CD90 Monoclonal Antibody (PE/Cyanine7 Conjugated) [5E10](AGEL1832)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)