Western blot analysis of extracts of various cell lines, using TIM44 antibody (CAB21141) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The TIM44 Monoclonal Antibody (CAB21141) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, produced in mice, exhibits high specificity and sensitivity for detecting TIM44 in various experimental settings such as Western blotting and immunofluorescence.TIM44 is a key player in the maintenance of mitochondrial function and has been linked to various human diseases including neurodegenerative disorders and metabolic syndromes. By targeting TIM44 with this antibody, researchers can gain valuable insights into the mechanisms underlying mitochondrial dysfunction and potentially identify novel therapeutic targets for these diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
TIM44 Monoclonal Antibody
SKU:
CAB21141
Size:
20μL, 100μL
Reactivity:
Human, Rat
Clone Number:
ARC3044
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TIM44
Positive Sample:
K562, Rat liver
Cellular Localization:
Fibrillar Center, Mitochondrial Inner Membrane, Mitochondrial Matrix, Mitochondrion.
Calculated MW:
51kDa
Observed MW:
45kDa
This gene encodes a peripheral membrane protein associated with the mitochondrial inner membrane translocase, which functions in the import of proteins across the mitochondrial inner membrane and into the mitochondrial matrix. The encoded protein mediates binding of mitochondrial heat shock protein 70 to the translocase of inner mitochondrial membrane 23 (TIM23) complex. Expression of this gene is upregulated in kidney in a mouse model of diabetes. A mutation in this gene is associated with familial oncocytic thyroid carcinoma.
Purification Method
Affinity purification
Gene ID
10469
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TIM44 Rabbit mAb (CAB21141) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
