The TIMM44 Antibody (CAB17446) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from rabbit serum, is highly specific to human samples and has been validated for use in various applications, including Western blotting. By targeting the TIMM44 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it essential for studies in mitochondrial biology and related fields.TIMM44 plays a vital role in the import of proteins into the mitochondria, a process essential for proper mitochondrial function and cellular metabolism.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TIMM44 Antibody
SKU:
CAB17446
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TIM44, TIMM44
Positive Sample:
U-87MG, K562, mouse liver, rat kidney
Cellular Localization:
Fibrillar Center, Mitochondrial Inner Membrane, Mitochondrial Matrix, Mitochondrion.
Calculated MW:
51kDa
Observed MW:
45kDa
This gene encodes a peripheral membrane protein associated with the mitochondrial inner membrane translocase, which functions in the import of proteins across the mitochondrial inner membrane and into the mitochondrial matrix. The encoded protein mediates binding of mitochondrial heat shock protein 70 to the translocase of inner mitochondrial membrane 23 (TIM23) complex. Expression of this gene is upregulated in kidney in a mouse model of diabetes. A mutation in this gene is associated with familial oncocytic thyroid carcinoma.
Purification Method
Affinity purification
Gene ID
10469
RRID
AB_2772612
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using TIMM44 Rabbit pAb (CAB17446) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunofluorescence analysis of L929 cells using TIMM44 Rabbit pAb (CAB17446) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
