The TJP2 Antibody (CAB0594) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is suitable for use in Western blot applications. By binding to the TJP2 protein, this antibody enables the detection and analysis of TJP2 expression in various cell types, making it ideal for investigations in cell biology and epithelial barrier function.TJP2, also known as ZO-2, plays a crucial role in maintaining the integrity and function of tight junctions, which are critical for regulating the passage of molecules across cell layers.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
TJP2 Antibody
SKU:
CAB0594
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a zonula occluden that is a member of the membrane-associated guanylate kinase homolog family. The encoded protein functions as a component of the tight junction barrier in epithelial and endothelial cells and is necessary for proper assembly of tight junctions. Mutations in this gene have been identified in patients with hypercholanemia, and genomic duplication of a 270 kb region including this gene causes autosomal dominant deafness-51. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene.
Purification Method
Affinity purification
Gene ID
9414
RRID
AB_2757285
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using TJP2 Rabbit pAb (CAB0594) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg TJP2 Rabbit pAb (CAB0594). Western blot was performed from the immunoprecipitate using TJP2 Rabbit pAb (CAB0594) at a dilution of 1:1000.
