Anti-TNFRSF11B Antibody (CAB2100)

Antibody Name:	Anti-TNFRSF11B Antibody
Antibody SKU:	CAB2100
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-280 of human TNFRSF11B (NP_002537.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, SW480, MCF7, NCI-H460, K-562, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 22-280 of human TNFRSF11B (NP_002537.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ETFP PKYL HYDE ETSH QLLC DKCP PGTY LKQH CTAK WKTV CAPC PDHY YTDS WHTS DECL YCSP VCKE LQYV KQEC NRTH NRVC ECKE GRYL EIEF CLKH RSCP PGFG VVQA GTPE RNTV CKRC PDGF FSNE TSSK APCR KHTN CSVF GLLL TQKG NATH DNIC SGNS ESTQ KCGI DVTL CEEA FFRF AVPT KFTP NWLS VLVD NLPG TKVN AESV ERIK RQHS SQEQ TFQL LKLW KHQN KDQD IVKK IIQD IDLC ENS
Gene ID:	4982
Uniprot:	O00300
Cellular Location:	Secreted
Calculated MW:	46kDa
Observed MW:	44kDa

Synonyms:

TNFRSF11B, OCIF, OPG, PDB5, TR1

Background:

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined.

UniProt Protein Function:	TNFRSF11B: Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis. Defects in TNFRSF11B are the cause of juvenile Paget disease (JPD); also known as hyperostosis corticalis deformans juvenilis or hereditary hyperphosphatasia or chronic congenital idiopathic hyperphosphatasia. JPD is a rare autosomal recessive osteopathy that presents in infancy or early childhood. The disorder is characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures, and deformities due to a markedly accelerated rate of bone remodeling throughout the skeleton. Approximately 40 cases of JPD have been reported worldwide. Unless it is treated with drugs that block osteoclast- mediated skeletal resorption, the disease can be fatal.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide; Inhibitor Chromosomal Location of Human Ortholog: 8q24 Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region Molecular Function:cytokine activity; receptor activity Biological Process: response to drug; extracellular matrix organization and biogenesis; response to magnesium ion; negative regulation of odontogenesis of dentine-containing teeth; response to estrogen stimulus; apoptosis; response to arsenic; negative regulation of bone resorption; signal transduction; skeletal development; response to nutrient Disease: Paget Disease, Juvenile
NCBI Summary:	The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	O00300
NCBI GenInfo Identifier:	322510101
NCBI Gene ID:	4982
NCBI Accession:	O00300.3
UniProt Secondary Accession:	O00300,O60236, Q53FX6, Q9UHP4, B2R9A8,
UniProt Related Accession:	O00300
Molecular Weight:	46,026 Da
NCBI Full Name:	Tumor necrosis factor receptor superfamily member 11B
NCBI Synonym Full Names:	tumor necrosis factor receptor superfamily, member 11b
NCBI Official Symbol:	TNFRSF11B
NCBI Official Synonym Symbols:	OPG; TR1; OCIF
NCBI Protein Information:	tumor necrosis factor receptor superfamily member 11B; osteoprotegerin; osteoclastogenesis inhibitory factor
UniProt Protein Name:	Tumor necrosis factor receptor superfamily member 11B
UniProt Synonym Protein Names:	Osteoclastogenesis inhibitory factor; Osteoprotegerin
UniProt Gene Name:	TNFRSF11B
UniProt Entry Name:	TR11B_HUMAN

	Western blot analysis of extracts of various cell lines, using TNFRSF11B antibody (CAB2100) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat spleen using TNFRSF11B Antibody (CAB2100) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human kidney cancer using TNFRSF11B Antibody (CAB2100) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using TNFRSF11B Antibody (CAB2100) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of HeLa cells using TNFRSF11B antibody (CAB2100). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-TNFRSF11B Antibody (CAB13250)	Human OPG (Osteoprotegerin) CLIA Kit (HUES00778)
Anti-TNFRSF11B Antibody (CAB16937)	Human Tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) ELISA Kit
TNFRSF11B Antibody (PACO01267)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)