Anti-TNRC6A Antibody (CAB6115)

Antibody Name:	Anti-TNRC6A Antibody
Antibody SKU:	CAB6115
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 950-1050 of human TNRC6A (NP_055309.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:1000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Rat brain

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 950-1050 of human TNRC6A (NP_055309.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QDIV GSWG IPPA TGKP PGTG WLGG PIPA PAKE EEPT GWEE PSPE SIRR KMEI DDGT SAWG DPSK YNYK NVNM WNKN VPNG NSRS DQQA QVHQ LLTP ASAI S
Gene ID:	27327
Uniprot:	Q8NDV7
Cellular Location:	Cytoplasm, P-body
Calculated MW:	17kDa/36kDa/182kDa/205kDa/206kDa/210kDa
Observed MW:	180kDa

Synonyms:

TNRC6A, CAGH26, GW1, GW182, TNRC6

Background:

This gene encodes a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The protein associates with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibiting expression of this gene delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing.

UniProt Protein Function:	TNRC6A: Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA- dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. Belongs to the GW182 family. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Translation; RNA-binding Chromosomal Location of Human Ortholog: 16p11.2 Cellular Component: cytosol; Golgi apparatus; intracellular membrane-bound organelle; nucleoplasm Molecular Function:protein binding Biological Process: miRNA-mediated gene silencing; miRNA-mediated gene silencing, negative regulation of translation; phosphoinositide-mediated signaling; RNA-mediated gene silencing; RNA-mediated posttranscriptional gene silencing; Wnt receptor signaling pathway, calcium modulating pathway
NCBI Summary:	This gene encodes a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The protein associates with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibiting expression of this gene delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing. [provided by RefSeq, Jul 2008]
UniProt Code:	Q8NDV7
NCBI GenInfo Identifier:	296452846
NCBI Gene ID:	27327
NCBI Accession:	Q8NDV7.2
UniProt Secondary Accession:	Q8NDV7,O15408, Q658L5, Q6NVB5, Q8NEZ0, Q8TBT8, Q8TCR0 Q9NV59, Q9P268, C9JAR8,
UniProt Related Accession:	Q8NDV7
Molecular Weight:	205,151 Da
NCBI Full Name:	Trinucleotide repeat-containing gene 6A protein
NCBI Synonym Full Names:	trinucleotide repeat containing 6A
NCBI Official Symbol:	TNRC6A
NCBI Official Synonym Symbols:	GW1; GW182; TNRC6; CAGH26
NCBI Protein Information:	trinucleotide repeat-containing gene 6A protein
UniProt Protein Name:	Trinucleotide repeat-containing gene 6A protein
UniProt Synonym Protein Names:	CAG repeat protein 26; EMSY interactor protein; GW182 autoantigen; Protein GW1; Glycine-tryptophan protein of 182 kDa
Protein Family:	Trinucleotide repeat-containing gene 6A protein
UniProt Gene Name:	TNRC6A
UniProt Entry Name:	TNR6A_HUMAN

	Western blot analysis of extracts of various cell lines, using TNRC6A antibody (CAB6115) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using TNRC6A antibody (CAB6115) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)